Dear all,
I am looking for an Ab for staining the intracellular part of CD8 alpha in FACS. Possible ? Any suggestions ?
Thx a lot,
Lennart
Im currently writing an essay about the analysis of applications of generative agents. I am about to start working on identifying potential or existing applications and analysing them. What would...
31 January 2024 2,317 0 View
Dear all, For culturing CTLL-2 cells, ATCC recommends to use CORNING T-STIM with Con A as a source for IL-2. However, delivery (for all distributors) would take almost two months and we...
04 January 2022 2,942 3 View
Hello. My name is Malte. In my master thesis I study assemblages of Meso and Makrofauna associated with arboreal soil in tree-cavities, following an approach of island biogeography. In my study...
07 July 2021 6,468 3 View
Dear all, I am looking for a way to up-regulate CD25 expression in CTL cells, but without activating them. Ideally we stimulate cells for the up-regulation of CD25 on day 1 and on day 2 CD25 is...
20 April 2021 4,708 0 View
Dear all, I am trying make stable clones of a HEK293 using an antibiotic selection strategy. However, the parental cell line is already resistant to the five commonly used antibiotics (G418,...
14 August 2020 7,693 2 View
Dear fellow EFL teachers, we, Laura Schmetzer and Lennart Holzhuetter, are currently writing our master's thesis on the topic of games in the EFL classroom and would really appreciate it, if you...
28 July 2020 3,230 2 View
Can you please help me identify the species of this Triturus? The photo is made in Netherlands.
28 June 2020 9,460 0 View
Basically, I need to model occurrence data and environmental layers in a MaxEnt program. Before doing so I need to prepare the environmental layers, i.e. convert them from netcdf to ascii, the...
30 April 2020 7,414 10 View
Hey community i have a question for you. Im doing a literature review and i want to put the relevant literature in an excel table to check it later. Whenever i am exporting something in CSV (using...
13 January 2020 6,284 3 View
Dear all, Anyone knows what the principle behind KCM transformation of bacteria is ? Thx, Lennart
06 February 2019 6,645 2 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...
07 August 2024 2,569 1 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks
02 August 2024 5,748 4 View