A typical pedigree.

01 January 2013 0 5K Report

We got a typical pedigree samples (n=10, and 4 patients). How to make a reasonable genetic analysis to search the candidate genes? With STR marker? SNP array? SNP+ CNV array? Exon sequencing? Given limited funding.

Badges
Science method

More Kejin Zhang's questions See All

Can anyone help me with a pedigree individual data analysis?

Dear All, I need your help. I have a pedigree and seven individuals included and whole genomic SNPs' genotypes. Now, I'm trying to analyze the linkage and linkage peak within whole genome with...

06 July 2014 1,173 12 View

How to verify the germline mutations using NGS?

Recently, I saw Bernd's work which analyses the difference between one MSI-CRC and MSS-CRC with NGS method. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3008745/). My interest is how to use Whole...

31 December 2012 3,505 5 View

How to deal with so many candidate genes?

A candidate gene's verification should be a excited news, but some time, it is a troublesome things for us. For example, for many genetic disease, especially the complex human diseases (e.g,...

31 December 2012 8,106 11 View

How to decide the suitable genetic program to use?

Hi, everybody. The software LAMP ( http://www.sph.umich.edu/csg/abecasis/LAMP/program_options.html) can be used in the linkage and association analysis like the other related programs, for...

10 November 2011 8,898 0 View

The freedom degree is 1/2?

Question: The freedom degree (df) number should be 1 or 2? when we analysis the distribution of certain gene's genotypes frequencies between case and control group by Chi2 test. As the...

09 October 2011 3,220 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

Are there always been barcodes, apapters and primer sequences in the FASTQ files of NGS?

Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...

20 July 2024 5,405 2 View

Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

Full Enquiry On Biomass Energy In engineering Technology?

How is biomass energy essential in the power generation in a country.

08 July 2024 9,477 2 View

Why does it show two bands when I add single hairpin structures to a gel?

Hi everyone, I am working on a hybridization chain reaction (HCR) and trying to visualize it with gel electrophoresis. The visualization of the HCR works but for the single components of this...

07 July 2024 9,413 4 View