Can anyone help me with a pedigree individual data analysis?

06 July 2014 1,093 12 View

How to verify the germline mutations using NGS?

Recently, I saw Bernd's work which analyses the difference between one MSI-CRC and MSS-CRC with NGS method. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3008745/). My interest is how to use Whole...

31 December 2012 3,421 5 View

How to deal with so many candidate genes?

A candidate gene's verification should be a excited news, but some time, it is a troublesome things for us. For example, for many genetic disease, especially the complex human diseases (e.g,...

31 December 2012 8,036 11 View

A typical pedigree.

We got a typical pedigree samples (n=10, and 4 patients). How to make a reasonable genetic analysis to search the candidate genes? With STR marker? SNP array? SNP+ CNV array? Exon sequencing?...

31 December 2012 4,476 0 View

The freedom degree is 1/2?

Question: The freedom degree (df) number should be 1 or 2? when we analysis the distribution of certain gene's genotypes frequencies between case and control group by Chi2 test. As the...

09 October 2011 3,150 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Statistical methods for comparing gene expression curves?

Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...

02 February 2021 2,613 7 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

New knockout bacterial strain: Can't electroporate it?

I made a new bacterial strain with an unmarked deletion using 2 step homologous recombination (with sacB counterselection). The full protocol is given in the link at the bottom. The protocol...

01 January 2021 1,580 4 View

Can you advise reference publications/standards on characterizing plant genetics research and development activities, and the outputs it generates?

I am doing semantic analysis of science and grey literature publications in the field of plant genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of art...

31 December 2020 5,871 4 View

What is the role of fresh cow dung in sprouting in stem cutting propagation?

In my personal experience I have find the higher rate of sprouting when fresh cow dung is applied on the top side of cutting what might be its reason.

24 December 2020 4,068 6 View

What is the level of consciousness , communication and feeling in plants ?

Do you think the plant can communicate with each other, what is the level of feeling in plants?

22 December 2020 2,754 17 View

How to cut dsDNA chain with Uracil-DNA Glycosylase at the point of dU in room temperature?

18 December 2020 6,908 3 View

Why pink coloured or light red onion have more storage life than white and dark red one and smaller size one to has more than bigger one?

It is generally seen light coloured varieties has more storage life.what's relation with colour and size with storage life.

13 December 2020 634 18 View

Can LAMP distinguish the species from different genus/family?

I am usiing LAMP kit, specifically designed for liriomyza huidobrensis. I have a random samples of differet insects and LAMP seems to amplify insects from other Genus as well. Although, other...

07 December 2020 5,630 3 View