• Hi, poison agar may work. Also include MIC. You can amend the mycelial growth test method by just replacing it with spore. All the best!

What is Poison Agar ? Can you please elaborate about your suggestions ?

Dear Maria

You can refer to my paper Nusaibah et al 2016, Plant Physiology and Biochemistry for the method.

best,

You can place the spore suspension in a 96 well plate with medium and different concentrations of inhibitor The spore suspension can then be incubated and screened in a microplate reader at several wavelengths, but commonly, between 400 and 600 nm. The more growth there is, the greater absorbance.

This method is used for determining IC50 and IC90 values in screening tests.

The plates may be prepared manually, but the best way is to use a liquid handling machine.These can be programmed to do this task very accurately.

For more information, search for fungal growth inhibition methods and high throughput screening.

Thank you, Unai. But this method evaluates growth inhibition. I need a method to evaluate spores being killed. 

Hi Maria

References 1

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=0ahUKEwiSzJmbyLTbAhWsAcAKHUxcC5YQFggqMAA&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F3938146&usg=AOvVaw0jztFkMgMGrNMfH1UfDyq-

2

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https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=0ahUKEwiSzJmbyLTbAhWsAcAKHUxcC5YQFggzMAI&url=http%3A%2F%2Feprints.hud.ac.uk%2F9633%2F1%2FHumphreysTesting.pdf&usg=AOvVaw0HjgZCoZZ_zJ-3kRZJE1CU

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https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=6&cad=rja&uact=8&ved=0ahUKEwiSzJmbyLTbAhWsAcAKHUxcC5YQFghBMAU&url=https%3A%2F%2Fwww.astm.org%2FStandards%2FE2197.htm&usg=AOvVaw3USGxNXJDXzbCL9YzGK7KU

5

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=7&cad=rja&uact=8&ved=0ahUKEwiSzJmbyLTbAhWsAcAKHUxcC5YQFghHMAY&url=http%3A%2F%2Fperfectusbiomed.com%2Fsectors%2Fdisinfectant-testing%2F&usg=AOvVaw1pgpUozN7Nx3dZLwb85W2n

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https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=8&cad=rja&uact=8&ved=0ahUKEwiSzJmbyLTbAhWsAcAKHUxcC5YQFghMMAc&url=https%3A%2F%2Fwww.pda.org%2Fdocs%2Fdefault-source%2Fwebsite-document-library%2Fchapters%2Fpresentations%2Fsoutheast%2Fdisinfectant-efficacy-testing-for-critical-environments.pdf%3Fsfvrsn%3D6&usg=AOvVaw30azDQjxezbRXnh8mis_Fj

7

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=9&cad=rja&uact=8&ved=0ahUKEwiSzJmbyLTbAhWsAcAKHUxcC5YQFghbMAg&url=https%3A%2F%2Fwww.tga.gov.au%2Fsites%2Fdefault%2Ffiles%2Fdisinfectants-evaluation-guidelines.pdf&usg=AOvVaw2yPmf1xp-7GBigkj974JDb

8

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=10&cad=rja&uact=8&ved=0ahUKEwiSzJmbyLTbAhWsAcAKHUxcC5YQFghpMAk&url=http%3A%2F%2Faem.asm.org%2Fcontent%2F75%2F17%2F5563.full&usg=AOvVaw1re5O35_qqEw8gZ6LhtWJy

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https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=13&cad=rja&uact=8&ved=0ahUKEwjtl6b98LTbAhURUlAKHWBkBZs4ChAWCDEwAg&url=http%3A%2F%2Fwww.oecd.org%2Fchemicalsafety%2Frisk-management%2F44256438.pdf&usg=AOvVaw3XrNqSrcr-6uqXREsiyEBW

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https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=14&cad=rja&uact=8&ved=0ahUKEwjtl6b98LTbAhURUlAKHWBkBZs4ChAWCDcwAw&url=http%3A%2F%2Fwww.ivami.com%2Fen%2Fbiocidal-activities-and-toxicology-with-disinfectants-25-accredited-tests%2F3318-b-bactericidal-fungicidal-and-sporicidal-b-nf-t72-190-1988-disinfectants-used-contact-in-liquid-water-soluble-method-germ-holder-b-enac-b&usg=AOvVaw2eCGL4rjZQlAIfJj68mNXL

11

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=15&cad=rja&uact=8&ved=0ahUKEwjtl6b98LTbAhURUlAKHWBkBZs4ChAWCDwwBA&url=http%3A%2F%2Fmicrochemlab.com%2Finformation%2Fintroduction-high-level-disinfectant-testing-0&usg=AOvVaw1vxStXUHrh7Dp0G2PJRhnM

12

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=16&cad=rja&uact=8&ved=0ahUKEwjtl6b98LTbAhURUlAKHWBkBZs4ChAWCEIwBQ&url=https%3A%2F%2Fwww.accuratuslabs.com%2Fen-1650-quantitative-suspension-test-for-the-evaluation-of-fungicidal-activity-of-chemical-disinfectants-3%2F&usg=AOvVaw0if1zuz_2RzRcg4GoFp1R6

13

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=17&cad=rja&uact=8&ved=0ahUKEwjtl6b98LTbAhURUlAKHWBkBZs4ChAWCEgwBg&url=https%3A%2F%2Fapvma.gov.au%2Fnode%2F1026&usg=AOvVaw0sLHaBKgwQlfhKospuVeDD

14

https://books.google.iq/books?id=KAakAgAAQBAJ&pg=PA657&lpg=PA657&dq=test+for+a+fungicidal/sporicidal+activity&source=bl&ots=KxfEMzIjUe&sig=5cAk2tofVjFnrmgGCxDAgpK8W_s&hl=en&sa=X&ved=0ahUKEwjtl6b98LTbAhURUlAKHWBkBZs4ChDoAQhRMAc

15

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=19&cad=rja&uact=8&ved=0ahUKEwjtl6b98LTbAhURUlAKHWBkBZs4ChAWCFMwCA&url=http%3A%2F%2Fonlinelibrary.wiley.com%2Fdoi%2F10.1111%2Fj.1365-2672.1983.tb02637.x%2Fpdf&usg=AOvVaw3_R7iZwYTbG1QUXAmixMpW

16

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=20&cad=rja&uact=8&ved=0ahUKEwjtl6b98LTbAhURUlAKHWBkBZs4ChAWCFkwCQ&url=https%3A%2F%2Fwww.sciencedirect.com%2Fscience%2Farticle%2Fpii%2FS0195670100908181&usg=AOvVaw1H0HLxqmi8BJC5gKc8iRcn

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https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=21&cad=rja&uact=8&ved=0ahUKEwi507fR_LTbAhWSJVAKHWTpAmI4FBAWCCYwAA&url=http%3A%2F%2Fsolutionsdesignedforhealthcare.com%2Fsites%2Fdefault%2Ffiles%2Fresources%2F1%2520-%2520A%2520product%2520based%2520on%2520accelerated%2520and%2520stabilized%2520hydrogen%2520peroxide_Evidence%2520for%2520broadspectrum%2520germicidal%2520activity.pdf&usg=AOvVaw1-du0jLykDpru1L4NBS70_

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https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=23&cad=rja&uact=8&ved=0ahUKEwi507fR_LTbAhWSJVAKHWTpAmI4FBAWCDAwAg&url=http%3A%2F%2Fsettek.com%2Fdocuments%2FEPA-Methods%2FPDF%2FEPA-Method-810-2100.pdf&usg=AOvVaw18P74mN03XxkrWNvmjtAlI

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=24&cad=rja&uact=8&ved=0ahUKEwi507fR_LTbAhWSJVAKHWTpAmI4FBAWCDUwAw&url=https%3A%2F%2Farchive.epa.gov%2Fpesticides%2Foppad001%2Fweb%2Fhtml%2Fcdif-guidance.html&usg=AOvVaw0kgPV6jMritgC-Wr_X_qaW

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=25&cad=rja&uact=8&ved=0ahUKEwi507fR_LTbAhWSJVAKHWTpAmI4FBAWCDowBA&url=https%3A%2F%2Fwww.frontiersin.org%2Farticles%2F10.3389%2Ffpubh.2018.00018%2Ffull&usg=AOvVaw0AReLzeSRppU_Ad1Kp02iQ