What does it mean when the MIC results are inconsistent amongst the replicates?

I am testing a particular antimicrobial to get its MIC using the microplate assay and the results I get are puzzling. In this specific case I have 8 replicates for each concentration but the MIC...

03 April 2018 255 10 View

Can anyone suggest a fun micro demonstration for kids!?

We have a BRING YOUR KIDS TO WORK DAY. Can anyone suggest a fun micro demonstration that kids (6-14 year olds) would enjoy? Thanks

01 February 2018 4,006 0 View

How to assess a synergy between water insoluble antimicrobial and solvent?

09 October 2017 9,606 4 View

A test for a fungicidal/sporocidal activity (mold spores)

I need to screen substances that would kill mold spores. Can someone recommend a test for a sporocidal activity? At the moment, I am challenging the spores with the tested substances and then...

07 August 2017 1,324 25 View

A question on buffer preparation technique

Recently I was preparing a citric acid buffer. Most literature describes the process of mixing citric acid and sodium citrate in a specific proportion. Is there a reason why I cannot just use...

06 July 2017 7,305 5 View

Buffer for microbial growth medium at pH 5.0-5.5

I need to run MIC experiments at pH 5.0 and 5.5. Can someone please suggest a buffering system that will work at these pH values, but won't kill the cells ? I want to add this buffer to acidified...

06 July 2017 6,326 5 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

Exit velocity of natural gas from pressurized tank?

Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...

26 February 2021 6,253 3 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View