What is the relationship between water activity of the solution and the osmotic pressure ?

It is known that low/high osmotic pressure can kill bacterial cells. I can easily measure water activity using equipment in my lab. Can this value be related to osmotic pressure using some...

06 July 2018 9,304 2 View

What does it mean when the MIC results are inconsistent amongst the replicates?

I am testing a particular antimicrobial to get its MIC using the microplate assay and the results I get are puzzling. In this specific case I have 8 replicates for each concentration but the MIC...

03 April 2018 255 10 View

Can anyone suggest a fun micro demonstration for kids!?

We have a BRING YOUR KIDS TO WORK DAY. Can anyone suggest a fun micro demonstration that kids (6-14 year olds) would enjoy? Thanks

01 February 2018 4,006 0 View

How to assess a synergy between water insoluble antimicrobial and solvent?

09 October 2017 9,606 4 View

A test for a fungicidal/sporocidal activity (mold spores)

I need to screen substances that would kill mold spores. Can someone recommend a test for a sporocidal activity? At the moment, I am challenging the spores with the tested substances and then...

07 August 2017 1,324 25 View

A question on buffer preparation technique

Recently I was preparing a citric acid buffer. Most literature describes the process of mixing citric acid and sodium citrate in a specific proportion. Is there a reason why I cannot just use...

06 July 2017 7,305 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View