I need to run MIC experiments at pH 5.0 and 5.5. Can someone please suggest a buffering system that will work at these pH values, but won't kill the cells ? I want to add this buffer to acidified TSB.
Please, have a look at pages 19 and 20 of this publication:
file:///C:/Users/V%C3%ADtek/Downloads/Calbiochem_Buffers_Booklet.pdf
Citric and acetate buzffers as well as citric-phosphate buffers are suitable biological buffers.
Best regards
Vit
Thank you, Vit! But I can't click on that link.
OK, try this one:
https://www.scribd.com/document/50270734/Buffers-Booklet-Calbiochem and afterwards click "download"
Regards Vit
OK, try the attachment, please.
Vit, thanks. I am surprised that citirc acid wouldn't kill the cells like sorbic or lactic acid at these pH values. Why is that?
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