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Questions related from Maria Zarina
It is known that low/high osmotic pressure can kill bacterial cells. I can easily measure water activity using equipment in my lab. Can this value be related to osmotic pressure using some...
07 July 2018 9,333 2 View
I am testing a particular antimicrobial to get its MIC using the microplate assay and the results I get are puzzling. In this specific case I have 8 replicates for each concentration but the MIC...
04 April 2018 284 10 View
We have a BRING YOUR KIDS TO WORK DAY. Can anyone suggest a fun micro demonstration that kids (6-14 year olds) would enjoy? Thanks
02 February 2018 4,031 0 View
Is there a method to assess synergy between a water insoluble antimicrobial and a solvent which is used to bring it to aquous solution ? I am very familiar with the Checkerboard assay, but to run...
10 October 2017 9,630 4 View
I need to screen substances that would kill mold spores. Can someone recommend a test for a sporocidal activity? At the moment, I am challenging the spores with the tested substances and then...
08 August 2017 1,365 25 View
I need to run MIC experiments at pH 5.0 and 5.5. Can someone please suggest a buffering system that will work at these pH values, but won't kill the cells ? I want to add this buffer to acidified...
07 July 2017 6,360 5 View
Recently I was preparing a citric acid buffer. Most literature describes the process of mixing citric acid and sodium citrate in a specific proportion. Is there a reason why I cannot just use...
07 July 2017 7,335 5 View