my result of PCR in electrophorosis can not run into the gel,all sample stay in the start line.what is the main couse?
May be the voltage you use is very low and can not be able to move the DNA from the wells
how to find a specific bacteria detail distribution through human bodys, like lung, skin, gut, intestine and eyes. Is there any powerful database that can solve this questions?
09 October 2016 7,109 8 View
We recently transformation pNL4,3 E-R- Luc+ plasmid(about 16kb) with Trans-1 competent cells(our lab made it). We picked ten more clone to perform digestion identification but all clones are...
31 December 2014 3,080 1 View
we need HBV genome perform HBV infection model but didn't have HBV genome plasmid who can give us some freely.......
08 September 2014 9,277 0 View
If my PEI doesn't work does it must account for wrong store method? Do you have any other suggestions to transfection with PEI?
07 August 2013 9,806 5 View
Recently we tried to transfect a plasmid into MDA-MB-231 and MCF-7 cell lines. After transfection we found that its transfection efficiency is different from other cell lines. MDA-MB-231 cell line...
31 December 2012 7,342 16 View
I cloned a 5Kb gene and linked into a common T vector which is about 3Kb. After screen with Amp i take it with normal PCR but the result of PCR is weired, there are two specific bands one is 3Kb...
03 April 2011 7,448 3 View
03 April 2011 4,300 2 View
hello every one, in gene targeting if the flank homology arm contains several mutaion does this dismatch affect recombination efficient? the whole sequence homology ratio can reach 90% also.
01 February 2011 299 3 View
hello,everyone.I'm construction a gene target vector.when I got the right flank sequence I found that the homology arm designed in a wrong direction.if I continue to follow original design I will...
31 December 2010 6,358 5 View
hello everyone! who can offer me a protocal about isolation stem cells from adipose and urine. any one who have papers about this can contact me with email or have a reply. my email address is...
07 August 2010 435 0 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...
17 July 2024 6,213 4 View
I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...
16 July 2024 4,604 0 View
I have conducted molecular marker amplification experiment by PCR and performed PAGE with silver staining. Now i have to score all my gels for further genetic analysis. Manual gel reading is...
12 July 2024 7,145 2 View
Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...
12 July 2024 2,058 5 View
I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...
09 July 2024 4,857 2 View