ask a protocal about stem cells isolation

08 August 2010 0 435 Report

hello everyone! who can offer me a protocal about isolation stem cells from adipose and urine. any one who have papers about this can contact me with email or have a reply. my email address is :[email protected]

thanks a lot !

Badges
Science topic

Similar topics
Cell Biology
Methods

More Liu Yuan wu's questions See All

How can I find a specific bacteria detail distribution through human body?

how to find a specific bacteria detail distribution through human bodys, like lung, skin, gut, intestine and eyes. Is there any powerful database that can solve this questions?

09 October 2016 7,109 8 View

Why transformation pNL4,3 E-R- Luc+ plasmids cannot pick up the right clone all clone?

We recently transformation pNL4,3 E-R- Luc+ plasmid(about 16kb) with Trans-1 competent cells(our lab made it). We picked ten more clone to perform digestion identification but all clones are...

31 December 2014 3,080 1 View

Who can gift us a pAAV/HBV1.2 plasmid

we need HBV genome perform HBV infection model but didn't have HBV genome plasmid who can give us some freely.......

08 September 2014 9,277 0 View

My PEI doesn't work at pH 7.0

If my PEI doesn't work does it must account for wrong store method? Do you have any other suggestions to transfection with PEI?

07 August 2013 9,806 5 View

Breast cancer cell line MDA-MB-231 transfection

Recently we tried to transfect a plasmid into MDA-MB-231 and MCF-7 cell lines. After transfection we found that its transfection efficiency is different from other cell lines. MDA-MB-231 cell line...

31 December 2012 7,342 16 View

Who can illustrate this consequence?

I cloned a 5Kb gene and linked into a common T vector which is about 3Kb. After screen with Amp i take it with normal PCR but the result of PCR is weired, there are two specific bands one is 3Kb...

03 April 2011 7,448 3 View

who can illustrate this phenomenon?

I cloned a 5Kb gene and linked into a common T vector which is about 3Kb. After screen with Amp i take it with normal PCR but the result of PCR is weired, there are two specific bands one is 3Kb...

03 April 2011 4,300 2 View

homologous recombination problem in gene targeting

hello every one, in gene targeting if the flank homology arm contains several mutaion does this dismatch affect recombination efficient? the whole sequence homology ratio can reach 90% also.

01 February 2011 299 3 View

Gene target vector construction problem

hello,everyone.I'm construction a gene target vector.when I got the right flank sequence I found that the homology arm designed in a wrong direction.if I continue to follow original design I will...

31 December 2010 6,358 5 View

A question about cryobiology

Hi everyone attention to this question! I found a doubt that some kind of fish can endure low temperature such as shrimp and Goldenfish.After freeze, it can be alive for a long time.If any one...

03 April 2010 5,088 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

Pragmatic inquiry research design?

Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...

28 July 2024 540 2 View

Can i apply a public questionnaire but analyse it with a different data analysis method?

First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...

27 July 2024 3,457 3 View

How to determine method detection limit in an analytical method?

I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...

24 July 2024 6,592 5 View

Do we put respondents data in research methods or findings section of a qualitative study?

Are both ways acceptable, or is there any convention to them? Thanks.

22 July 2024 6,988 4 View

Restaurant research, methods of quantitative and qualitative statistics?

Greetings! I am looking for materials on quantitative and qualitative research. What are the methods of collecting information and the principles of their implementation? I would like to make an...

20 July 2024 2,033 7 View