12 Questions 23 Answers 0 Followers
Questions related from Liu Yuan wu
how to find a specific bacteria detail distribution through human bodys, like lung, skin, gut, intestine and eyes. Is there any powerful database that can solve this questions?
10 October 2016 7,084 8 View
We recently transformation pNL4,3 E-R- Luc+ plasmid(about 16kb) with Trans-1 competent cells(our lab made it). We picked ten more clone to perform digestion identification but all clones are...
01 January 2015 3,051 1 View
we need HBV genome perform HBV infection model but didn't have HBV genome plasmid who can give us some freely.......
09 September 2014 9,252 0 View
If my PEI doesn't work does it must account for wrong store method? Do you have any other suggestions to transfection with PEI?
08 August 2013 9,769 5 View
Recently we tried to transfect a plasmid into MDA-MB-231 and MCF-7 cell lines. After transfection we found that its transfection efficiency is different from other cell lines. MDA-MB-231 cell line...
01 January 2013 7,305 16 View
I cloned a 5Kb gene and linked into a common T vector which is about 3Kb. After screen with Amp i take it with normal PCR but the result of PCR is weired, there are two specific bands one is 3Kb...
04 April 2011 4,273 2 View
04 April 2011 7,419 3 View
hello every one, in gene targeting if the flank homology arm contains several mutaion does this dismatch affect recombination efficient? the whole sequence homology ratio can reach 90% also.
02 February 2011 268 3 View
hello,everyone.I'm construction a gene target vector.when I got the right flank sequence I found that the homology arm designed in a wrong direction.if I continue to follow original design I will...
01 January 2011 6,324 5 View
hello everyone! who can offer me a protocal about isolation stem cells from adipose and urine. any one who have papers about this can contact me with email or have a reply. my email address is...
08 August 2010 399 0 View
my result of PCR in electrophorosis can not run into the gel,all sample stay in the start line.what is the main couse?
04 April 2010 1,789 1 View
Hi everyone attention to this question! I found a doubt that some kind of fish can endure low temperature such as shrimp and Goldenfish.After freeze, it can be alive for a long time.If any one...
04 April 2010 5,045 2 View
