hello,everyone.I'm construction a gene target vector.when I got the right flank sequence I found that the homology arm designed in a wrong direction.if I continue to follow original design I will get a target vector that must contain a loop in the homologous recombinataion event,dose this affect efficient of targeting seriously?
Hey can you tell me on which software you are constructing vector ??
What will be procedure if you are introducing "New Gene or sequence" [ specially standard software ] through virus ?
Thanks Rohit and Mehvish,my problem now isn't how to construct a targeting vector.the main question is that the target vector which I designed in a wrong directiion will fomation a loop when homologous recombination experiment.In standard protocal this condition didn't described and i didn't find any reference to comment this.
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