hello every one, in gene targeting if the flank homology arm contains several mutaion does this dismatch affect recombination efficient? the whole sequence homology ratio can reach 90% also.
Brad Feilmeier
Depends on the organism. Neisseria and Strep don't have much problem. Anything else, probably.
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Stephan A. Frye
Even in the Neisseriacea there are big differences and 10% sequence difference can drop the transformation frequency by several log10.
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