How can I find a specific bacteria detail distribution through human body?

09 October 2016 7,055 8 View

Why transformation pNL4,3 E-R- Luc+ plasmids cannot pick up the right clone all clone?

31 December 2014 3,017 1 View

My PEI doesn't work at pH 7.0

If my PEI doesn't work does it must account for wrong store method? Do you have any other suggestions to transfection with PEI?

07 August 2013 9,741 5 View

Breast cancer cell line MDA-MB-231 transfection

Recently we tried to transfect a plasmid into MDA-MB-231 and MCF-7 cell lines. After transfection we found that its transfection efficiency is different from other cell lines. MDA-MB-231 cell line...

31 December 2012 7,272 16 View

Who can illustrate this consequence?

I cloned a 5Kb gene and linked into a common T vector which is about 3Kb. After screen with Amp i take it with normal PCR but the result of PCR is weired, there are two specific bands one is 3Kb...

03 April 2011 7,375 3 View

who can illustrate this phenomenon?

I cloned a 5Kb gene and linked into a common T vector which is about 3Kb. After screen with Amp i take it with normal PCR but the result of PCR is weired, there are two specific bands one is 3Kb...

03 April 2011 4,244 2 View

homologous recombination problem in gene targeting

hello every one, in gene targeting if the flank homology arm contains several mutaion does this dismatch affect recombination efficient? the whole sequence homology ratio can reach 90% also.

01 February 2011 241 3 View

Gene target vector construction problem

hello,everyone.I'm construction a gene target vector.when I got the right flank sequence I found that the homology arm designed in a wrong direction.if I continue to follow original design I will...

31 December 2010 6,294 5 View

ask a protocal about stem cells isolation

hello everyone! who can offer me a protocal about isolation stem cells from adipose and urine. any one who have papers about this can contact me with email or have a reply. my email address is...

07 August 2010 364 0 View

A question about cryobiology

Hi everyone attention to this question! I found a doubt that some kind of fish can endure low temperature such as shrimp and Goldenfish.After freeze, it can be alive for a long time.If any one...

03 April 2010 5,007 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View