A molecule inhibits both single and double standard binding proteins. What could be the most probable mode of action of this molecule. Protein or DNA?

19 October 2022 0 3K Report

A molecule inhibits both single and double-standard binding proteins. What could be the most probable mode of action of this molecule? Protein or DNA? What are the possible methods/ experiments to validate this?

Kindly share your experience!!

Badges
Science topic

More Biswajit Naik's questions See All

Residuals are getting converged, but at a very migh magnitude of values. Why??

I am running a coarse DNS case for pipe flow with 2.1 Million cells. My residuals are quite fluctuating as its a fully turbulent annular pipe flow case but its getting statistically converged to a...

29 May 2024 2,063 5 View

Effect of \nu variation for a turbulent pipe flow at a constat Reynolds number ???

I am trying to run a turbulent pipe flow simulation with turbulent Reynolds number of 600, for 15 seconds, the flow is pressure driven due to gravity enforced buoyancy force which gives kinematic...

20 May 2024 5,619 3 View

Why <u'v'> cross-corelation is important whilte studying Reynolds Stresses in most studies??

In most common turbulence model studies, when considering Reynolds stresses the components of auto-correlation, , , and components of cross-correlation are considered. In the research studies...

02 May 2024 2,776 2 View

Could you please suggest me some of the best multi-parameter water analyzer kits (with brand) that can be taken to field for water quality sampling?

I am planning to work on different water bodies of Andhra Pradesh. So I want to purchase a portable water testing kit with multiple parameters. Please suggest me a few best option, which would be...

17 April 2024 7,210 3 View

Enforcing Wall shear stress (\tau_w) as a boudnary condition in OpenFOAM?

I am working on WMLES (wall Modelled LES) for which if I calculate my wall shear stress analytically and want to enforce it as a boundary condition at the wall patch, so that I do not need to...

15 April 2024 9,059 3 View

Relation between Bulk (Mean) and Friction Reynolds Number??

Is there any specific relation between Re_b (bulk/ Mean Reynolds number) and Re_\tau (friction Reynolds Number). In few of the literature and experimental work I have gone through researchers give...

01 April 2024 861 5 View

How can I make this type road network connectivity map in ArcGIS software?

How can I make this type road network connectivity map in ArcGIS software? please help me Please see the map .

10 March 2024 8,483 2 View

Why are the iterations needed to calculate final converged value of Yplus for wallFunctions in OpenFOAM?

This is a code block from nutWallFunction library in OpenFOAM where in, effective kinematic viscosity ($\nut_w$) at the wall is calculated using resolved field(in case of LES)/ mean field(in case...

04 March 2024 9,548 2 View

Effect of Prandtl on velocity for Natural Convection (Rayleigh Benard)?

When Prandtl number is increased for two cases tested over same Rayleigh number, the peak vertical-velocity decreases as Prandtl is increased. This is questionable to the fact that in general...

11 February 2024 4,196 2 View

How to calculate y+ analytically before the simulation if we have fully developed flow for the Annular pipe (a double wall bounded)?

I have a fully developed pipe flow in with Inner radius (r) and outer radius (R), using pressure driven flow condition due to buoyancy, - (1/rho) dP/dx = g and velocity scaling u* = sqrt ( (-R/...

09 February 2024 3,727 3 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

How can CHARMM-GUI be used for protein-ligand interaction ?

My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.

24 June 2024 5,044 1 View

How to predict Protein structure and choose N or C terminal tagging?

Hi, may I know I want to fuse my protein with affinity tag for protein purification in Expi293, how can I determine and visualise how does a N or C terminal tag affect my proteins (structure,...

24 June 2024 8,622 6 View

How to incease the yiald of recombinant protein expression ?

Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...

12 June 2024 9,616 8 View