From your experience - what are the biggest impediments in the implementation of Digital Twins?

Even almost 20 years after the first Digital Twin (DT) concept was presented by NASA, there is still no broad implementation of DTs in industry is known to date. Why is that? What is hindering...

29 October 2020 1,003 7 View

Do you know of any publication manuals or style guides that have a chapter on bias-free / inclusive language, aside from APA (7th edition)?

The Publication Manual of APA (7th edition) has a very useful chapter on bias-free language. I would like to know if you've come across such chapters or sections in other publication manuals or...

21 October 2020 1,582 4 View

What can I do to get a straight baseline in RP-HPLC?

I am running a RP-HPLC system with a C18 column. In our group, we mainly use the system to analyze peptides. The baseline of the system is not stable. In every run it is increasing over time. The...

29 July 2020 5,003 11 View

What is the optimal well surface area for C2C12 myoblast differentiation into myotubes?

Does anyone know of studies investigating the optimal well sizes for growth/hypertrophy of C2C12 myoblast-derived myotubes following differentiation using standard serum starvation? I find that...

06 July 2020 598 2 View

Which tube should I use to connect vacuum oil pump with Schlenk line?

I want to connect the vacuum oil pumps in our lab with the Schlenk lines and I am not sure which tubes (material, brand, etc.) I should choose. At the moment I am using a red rubber tube and it is...

30 January 2020 7,354 2 View

Superficiality or shallowness scales for trait evaluation?

Does anyone know if there exist a psychometric scale that measures the personal shallowness/superficiality of an individual when evaluating other's individual traits (age, race, gender,...

11 September 2019 2,566 3 View

How to calculate inter-coder reliability (two coders) with observations fitting almost into the same category?

02 June 2019 979 2 View

How to attach specific bacteria to the surface of micro-spheres/beads?

We are starting up a new project where it would be highly relevant to attach specific bacteria to the surface of micro-spheres/beads of e.g. alginate. However, literature and potential...

18 April 2018 7,993 2 View

Can I selectively remove host cells from mouse blood while retaining intact bacteria?

To enumerate the total amount of bacteria per blood volume, I would like to selectively remove host cells from mouse blood while retaining bacteria. Size filtration is unlikely to work as filters...

22 October 2017 6,278 3 View

Preservation of bacterial transcriptome upon cooling?

Is there any study around on how well bacterial transcriptomes are preserved when they are taken out of the "in vivo" situation and kept on ice for varying amounts of time? In other words, how...

12 September 2017 2,450 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View