Does a parents education level have an impact on their child's education?

Do children follow in the same footsteps as their parents in terms of their education?

12 January 2021 1,375 7 View

ANSYS, how is far-field acoustic pressure calculated (from which underlying surface)?

In ANSYS, as in also e.g. COMSOL Multiphysics, you can calculate a far-field acoustic pressure outside the explicit FEM domain. However, whereas in COMSOL Multiphysics you define a certain closed...

04 January 2021 3,076 8 View

Protocol for activating PBMCs using PHA/IL-2?

Hi there, just looking for a simple protocol stimulating 1 x 10^6 PBMCs/ mL in RMPI + 10%FBS using PHA and IL-2? I am looking for CD25, NKp44, c-kit expression and a few other markers for flow...

08 December 2020 2,087 1 View

Any advice on how to get KO cell lines by santa cruz Crispr-cas9 please?

Hello, I tried several times to obtain two KO cell lines for ERN1 (IRE1 alpha) using a santa cruz crispr cas9 "double nickase". (HNSCC cancer cell lines : PECA-PJ41 and PECA-PJ34). I tried...

07 December 2020 8,141 1 View

after dissolving biomass in an ionic liquid and filtering it with denoised water. how may i find out how much of cellulose in my filtrate?

i was wondering if you know of an accurate method for quantifying the amount of cellulose in a filtrate. of biomass that had been dissolved in ionic liquid and then filtered with deionized...

05 November 2020 6,469 2 View

Protocol for fixing mouse livers and fat pads for H&E staining?

Hi there! I need advice on a protocol for mouse tissue staining. I will dissect mouse livers and fat pads and look for liver steatosis and adipocyte perimeter. I was planning on doing this with...

22 November 2019 7,490 6 View

Why do my KGN granulosa cells are shrinking when treated with PMA ?

Hello everyone I need help, I am working with KGN granulosa cell line (DMEM/F12 media, 10% FBS, 1% Pen/Strep) I am treating the cells with PMA at 100nM concentration to activate PKC, but...

29 April 2019 5,057 3 View

Which statistical test(s) should be run with ordinal data?

The research has: 1 IV and 3 DV's. Likert scales used yielding scores of 1-5 on Behavioural Observational Scales (BOS). n=99 with each individual being scored on all 3 DV's. Total BOS=297. 99 BOS...

30 November 2018 8,807 4 View

Which statistical test(s) should be run with what kind of data (nominal, ordinal, etc)?

1 IV and 3 DV's. Likert scales used yielding scores of 1-5 on Behavioural Observational Scales (BOS). n=99 with each individual being scored on all 3 DV's. Total BOS=297. 99 BOS scored for each...

29 November 2018 6,231 4 View

What is the best method of alkaloid extraction from insects?

I'm working on a project for which I need to extract the alkaloids in Lycorma Delicatula (the spotted lanternfly) for HPLC, and I'm having a difficult time finding an adequate protocol for...

14 November 2018 9,004 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View