Can the 4T1 metastatic breast cancer model - which is a syngeneic xenograft model based on 4T1-12B - be used as a model to study the anticancer effects of plant based compounds without injecting into a balb/c mice?
Can one culture it directly in flasks, apply plant based extracts and study the effects of plants on the cells?
I'm not sure which thread is the original one, but I'll re-emphasize my answer here; in vitro cell lines of murine origin will be better for compatibility purposes when translating in vitro cytotoxicity results into drug dosages for 4T1 xenografts (see our examples here: http://altogenlabs.com/xenograft-models/breast-cancer-xenograft/4t1-xenograft-model/). However, because 4T1 is of murine origin, the in vivo results may differ significantly from a study done on a human cell line like MCF-7.
If it is a plant based therapeutic, or diet based, you need to give it orally. If it is an identified compound IP is OK.
If you want to do in vivo work (which is the point of a metastatic model) you must use Balb/c mice. However, it will grow in vitro.
The answer is yes, you can culture 4T1 cells in dishes and flasks, and you can apply any type of compounds regardless of whether it is plant based extracts or not. We did similar experiments, so please see our paper for details. Nagahashi M, Takakbe K et al, Cancer Res 2012;72:726-735.
Dear Stephen, you are correct. 4T1 cells grow very well in culture using such medium as DMEM (4g/L glucose) + ultraglutamine + 10% FCS. You will be able to add your drugs/compounds of choice to the cell culture medium and monitor activity in a variety of ways. However, be aware that 4T1 is not a xenograft model; these are murine tumour cells transplanted into mice, therefore they are syngeneic.
To answer your question, yes, the cell line can be cultured in a flask to study the anticancaer effct of your compound of interest. However, eventually you need to move into in vivo models and for that you need to develop a formulation which can be used for animal treatment.
Kazuaki Takabe.....i am not able to trace your article.
Can you send a copy at [email protected]
Regards
Thanks Takabe. I am going through it now. I have sent you an email seeking more assistance.
Line 4T1 is a thioguanine-resistant variant that was selected from 410.4 without mutagen treatment. Line 4T1 spontaneously metastasizes to both the lung and liver as evidenced by the formation of visible nodules in these organs in mice bearing line
4T1 primary tumors.
Selective Events in the Metastatic Process Defined by Analysis of the Sequential
Dissemination of Subpopulations of a Mouse Mammary Tumor1
Cheryl J. Aslakson and Fred R. Miller CANCER RESEARCH 52, 1399-1405, March 15, 1992]
The 4T1 is a murine cell line, so it is not a human cancer. Maybe, inoculation of cell line of human breast cancer in athymic mice (Balb/c nu/nu) could be contribute with a more precise results to test these compounds.
Denise Priolli...thanks for your input. I take note that 4TI is a murine cell line.
From your side, is this cell line a murine breast cancer model which can display cancer properties in vitro?
Murine cell line would show properties of murine cancer. Because of this, I think more adequate the cell line of human breast cancer. Of course, it depends of the research aim.
I'm not sure which thread is the original one, but I'll re-emphasize my answer here; in vitro cell lines of murine origin will be better for compatibility purposes when translating in vitro cytotoxicity results into drug dosages for 4T1 xenografts (see our examples here: http://altogenlabs.com/xenograft-models/breast-cancer-xenograft/4t1-xenograft-model/). However, because 4T1 is of murine origin, the in vivo results may differ significantly from a study done on a human cell line like MCF-7.
Dr Talmadge's answer is corect. 4Ti grows both in vitro and in Balb/c mice. It is a murine tumor. I don't know where the number 128 came from
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