Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant... | Contact experts in Solvents to get answers
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Questions related to Solvents
I am using HEK293 cell line and following this protocol from...
10 February 2025 7,401 1 View
I am new to using AMBER. My pdb id of the protein is 2beg. Also, in simulating this protein with a ligand, do I need to remove the hydrogen atoms of the complex and protein before running in tleap...
06 February 2025 1,207 3 View
I am currently developing a novel coating for titanium implants, and I have encountered some challenges with one of my experimental groups. In this group, I used electrodeposited calcium phosphate...
05 February 2025 1,243 1 View
Non crystalline materials
04 February 2025 9,562 3 View
The extraction was made according to Aebi 1984. A complete reaction mixture contains 1.5 ml of 100 mM potassium phosphate buffer (pH 7.0), 0.5 ml of 75 mM H2O2, 0.2 ml of enzyme extract and 0.8 ml...
02 February 2025 7,396 2 View
The extraction was made according to Aebi (1984). A complete reaction mixture contains 1.5 ml of 100 mM potassium phosphate buffer (pH 7.0), 0.5 ml of 75 mM H2O2, 0.2 ml of enzyme extract and 0.8...
02 February 2025 7,201 2 View
The scientific name of the Rudraksha plant is Elaeocarpus ganitrus. It is considered important in Hindu culture as the dried seeds of this plant, known as Rudraksha beads, are used as prayer beads...
30 January 2025 5,032 4 View
protein sample should be intact in -70c as far as I know. but when I stored them 1 week after first western blot, I can't even detect b-actin signal in the second experiment. I used cold RIPA...
27 January 2025 279 2 View
For photosynthesis determinations in plants under freezing stress (-6 oC), what is the correct setup of the IRGA? especially the temperature for the determination.
27 January 2025 9,951 2 View
Hello! I am developing a protocol for extracting the extracellular polymeric substances from microbial biofilms using cation exchange resin (CER). Many publications exist, however I am not able to...
27 January 2025 7,075 1 View
Suppose you began your career in organic chemistry (MSc and PhD) but later specialized in analytical chemistry (Dr. Sci.) and conducted research in this field for over 15 years. How would you...
26 January 2025 5,803 9 View
Im shooting 100ul of a 1mg/ml pure sample with no column into an Agilent 1100 HPLC using a detector at 210nm, flow rate 0.6ml/min, 35%ACN, 0.15%FA:65%H2O, 0.15%FA and getting split peaks, what...
22 January 2025 1,299 13 View
hello. I fixed B16F10 cells with FA 5% for 5 min and blocked with BSA 1% for 1h. I then stained them with Lysotraker red DND-99 from invitrogen at various ratios, and LAMP-1 (sc-20011) to make...
18 January 2025 5,355 0 View
I want to optimize a repeating unit of pectin instead of the whole pectin.
18 January 2025 7,886 0 View
I am conducting a ROS experiment by infecting RAW cells with Staphylococcus aureus to induce ROS production, using H2DCFDA for detection. Is it feasible to use a 96-well plate with an MOI of 1 for...
15 January 2025 8,454 0 View
I came across a young Prosopis juliflora plant, which is highly invasive. What is the best method for collecting sand around the roots to study the potential
09 January 2025 5,877 5 View
This is my recent work on aspen hysys. I don't have advance knowledge about aspen hysys and aspen plus.
07 January 2025 2,226 2 View
Hello, I am attempting to create a BSIM CMG SPICE model using Keysight Device Modeling MBP. I am focusing on a specific GAAFET device with fixed dimensions, and I plan to use version 108. From...
02 January 2025 886 0 View
I am conducting research on spiropyran for its potential applications in self-reporting materials. However, I am facing challenges in dissolving spiropyran in a solvent without compromising its...
26 December 2024 8,980 0 View
please also recommend some papers for me to read to better understand the problem
25 December 2024 1,573 1 View
When i use this catalyst at pH 3 and 11 it showed better performance. When i use it in the KHCO3 reaction solution instead DI water the trend of absorbance was inverse. please guide me what could...
25 December 2024 6,052 0 View
Dear friends, I am trying to preparing the ready to press (RTP) granules for silicon nitride ceramics. Typically, ethanol is used as the solvent, and ball milling is used for mixing and...
06 December 2024 4,880 2 View
Hello! I requested a paper from an author, and they responded (yay)! However, I cannot read the full message they sent with the paper; I can only see the preview. I would like to read the message...
06 December 2024 5,933 3 View
I am working on proteomics. Due to non-availability of nanoLCMS, I have conducted a ESI-MS analysis of a test sample. I have identified the proteins using XTandem. I am planning to quantify...
06 December 2024 4,857 2 View