Ammar Bader Sir,

The accepted name for Prosopis juliflora is Neltuma juliflora.

https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:509505-1

Thanks!

You could do it like the archeologists: Use shovel and buckets and take out layer after layer.

When studying allelopathic compound secretion from the roots of Prosopis juliflora, collecting sand around its roots requires careful techniques to avoid contamination and preserve the chemical integrity of the samples. Below is a recommended method:

Best Method for Collecting Sand Around Prosopis juliflora Roots

1. Preparation

• Objective Focus:The goal is to collect sand from the rhizosphere (the soil immediately surrounding the roots) where allelopathic compounds are most concentrated.
• Timing:Collect samples early in the morning when temperature fluctuations are minimal to reduce compound volatilization. Ensure the soil is moist, as dry conditions can make sampling more challenging.
• Tools and Materials:Sterile tools: Small shovel, trowel, or soil auger. Clean, airtight containers (glass or high-quality plastic) for sample storage. Gloves to prevent contamination. pH meter or test kit (optional) for onsite pH assessment. Cooler with ice packs for immediate transport to minimize chemical degradation.

2. Collection Procedure

• Identify the Collection Zone:Focus on the rhizosphere, ideally within 1–3 cm of the root surface. Expose fine feeder roots where the secretion of allelopathic compounds is most active.
• Careful Excavation:Begin by removing debris and loose topsoil from around the plant. Dig carefully in a circular pattern to expose the root zone without damaging the roots.
• Sand Removal:Use a sterile brush or small hand tool to gently collect sand directly adhering to the roots. Avoid mixing sand from the bulk soil (farther away from the roots) with rhizosphere samples.

3. Sample Handling

• Immediate Sealing:Place collected sand directly into airtight containers to prevent exposure to air, light, or moisture changes. Avoid using bags, as they may allow moisture exchange and affect compound stability.
• Labeling: Record details including:Date and time of collection. Location (GPS coordinates recommended). Plant age or size and environmental conditions. Observed soil texture, color, or odor (if relevant).
• Transport:Store containers in a cooler with ice packs if immediate lab analysis is not possible. Minimize transit time to preserve the integrity of allelopathic compounds.

4. Laboratory Analysis

• Chemical Extraction:Use solvents like methanol, ethanol, or distilled water to extract allelopathic compounds from the sand. Employ methods such as high-performance liquid chromatography (HPLC) or gas chromatography-mass spectrometry (GC-MS) for compound identification.
• Controls:Collect sand samples from an area away from Prosopis juliflora roots as a control to compare compound concentrations.

Key Considerations

• Avoid Cross-Contamination:Sterilize tools between samples and avoid touching roots or sand with bare hands.
• Minimize Compound Loss:Keep samples cool and shielded from sunlight to prevent degradation of volatile allelopathic compounds.
• Environmental Factors:Document surrounding vegetation, soil type, and water availability, as these can influence allelopathy.

The method for collecting sand around Prosopis juliflora roots to study allelopathic compound secretion is informed by established research in rhizosphere sampling and allelopathy studies. Key sources include:

Thank you very much for your efforts and helpful reply.