The extraction was made according to Aebi (1984). A complete reaction mixture contains 1.5 ml of 100 mM potassium phosphate buffer (pH 7.0), 0.5 ml of 75 mM H2O2, 0.2 ml of enzyme extract and 0.8 ml of distilled water was used. In case of reading on Spectro at 240nm no linear decrease was observed despite continues up down was noted in the values. What could be the possible reason? How we can come up with??