Seeds

The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like... | Review and cite SEEDS protocol, troubleshooting and other methodology information | Contact experts in SEEDS to get answers

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Questions related to Seeds

What camera to use for seed dispersal measurement using image processing?

Hello, I am going to do experiments on seed dispersal of Arabidopsis thaliana in a wind tunnel. As its seeds are super small and can come in a large number, I will later use image processing for...

30 January 2023 2,425 2 View

How to prepare alpha MEM culture medium?

I want to prepare culture medium for osteoblast. I could not find the proper protocol in internet. Kindly help me

20 January 2023 2,134 0 View

Alternatives or more accurate methods of proximate analysis of isolates and concentrates?

Hi, I'm currently working on insect-based products. All the methods of quantifying carbohydrates, proteins, fibers, fats etc that I have come across with so far are by proximate means. Has anyone...

19 January 2023 3,570 3 View

Ma-mel-66a cell line culturing methods?

Hello, I have been trying to passage ma-mel-66a cell line, however, I am having zero luck. I have used RPMI + 10%FBS and RPMI + 5%FBS, I even plated them (straight from LN2) in a small p10 to...

11 January 2023 9,271 5 View

How to spread silica, PVP and silicon oil solution well in blood clot activator tube?

Hi I have a question about the blood clot activation (BCA) tube (under vacuum). To make this pipe, we spray silica(solve in ethanol) + polyvinyl pyrrolidone(solve in ethanol) + silicone oil...

10 January 2023 6,797 0 View

Why are the current and voltage signals too noisy in case of dielectric barrier discharge plasma using line frequency?

I am using 50 Hz, DBD plasma for material treatment. I see that the current and the voltage signals are too noisy. What is the reason behind it? How can i eliminate this noise? How would such...

22 December 2022 2,120 3 View

Can someone identify these fish parasites?

These were collected from the intestines of Tilapia (Oreochromis spp.) and viewed under the microscope (magnification: 4x).

22 December 2022 5,084 4 View

How to extract sesamin and sesamolin from sesame cake?

We do a treatment for sesame seeds in order to separate the crust(cake) of sesame seeds and get the core to be ground to get tahini. the question now I have a large quantity of this crust and I...

19 December 2022 9,194 0 View

How to quickly confirm there are histone modification in a gene?

How do I know if there is a method like predictive net to detect histone acetylation modifications (e.g. H3K9ac or H4K5ac) in target genes before the experiment? I am a new student in histone...

18 December 2022 7,286 0 View

What are the units of Harvest index, Stover yield and Seed output (yield parameters) of any crop?

What are any crop's units of Harvest index, Stover yield, and Seed output (yield parameters)? in the case of the pea (Pisum sativum)crop

13 December 2022 2,590 3 View

To sterilize the tiny seeds of Habenaria dentata, could I get some advice?

I am afraid that I will kill the seeds by crutial concentration of EtOH and NACIO. May I get some good protocol or information?

09 December 2022 5,221 3 View

What if there is multicollinearity issue?

Hello everyone. I wanted to use rolling regression analysis , but i have multicollinearity issue and i do not want to delete variables as i only have 9 variables. Any suggestions? thank you

29 November 2022 8,901 8 View

How to solve autocorrelation and multicollinearity problems in ARDL model by using STATA?

To do research paper I am using ARDL model when I run the model my data, which is Annual time series data, has autocorrelation and multicollinearity problems. My Obs. 30 years.

28 November 2022 9,227 1 View

What is the lentiviral titer amount and what's the puromycin conc. to use for ARPE-19 transduction?

I am trying to transduce ARPE-19 cell line. I used Lipofectamine 3000 to produce my lentiviral vectors. My 1st attempt at transduction is unsuccessful. I have seeded 0.3 million cell per well in...

17 November 2022 4,429 0 View

For Basque speaking researchers: How has the concept "Grounded Theory" been translated into our language?

Kaixo! Marina Landa naiz, nire tesia euskaraz idazten ari naiz eta jakin nahi nuke ea badagoen "Grounded Theory" kontzeptuaren itzulpen zehatzik gure hizkuntzan. Eskerrik asko! Hi! My name is...

11 November 2022 7,413 2 View

¿Research centers working with Hemp in Portugal?

¿Do someone know any research institution working with hemp in Portugal?

09 November 2022 4,505 0 View

How to prevent cells from adhering while calculating seeding volume?

Hey Guys I am working on HeLa cells and I need to do a clonogenic assay. Following are the steps I follow for seeding 1. Remove media and wash with 1XPBS 2. Add trypsin and incubate for 1...

08 November 2022 1,119 0 View

Is plant authentication is important??

I did not want to authenticate my plant sample so Will this cause problems for me to publish papers in the future? Manily, i have worked with one plant but now the problem is the tree is no...

06 November 2022 4,542 1 View

Incubation study of soil and biochar and changes in soil EC (electrical conductivity) throughout the incubation period?

Incubation study of soil and biochar and changes in soil EC (electrical conductivity) throughout the incubation period.

03 November 2022 9,185 6 View

Why am I getting negative values on my cell viability assay?

Hi, I'm using the alamarBlue reagent to continue testing small-molecule drugs on glioblastoma cells. I seed 62500 cells on 96 well plates. I draw a standard curve first. I go from 500000 cells...

26 October 2022 2,747 0 View

Can anybody suggest a best protocol for culturing primary cortical neurons from post-natal rat pups?

I have been working on culturing primary cortical and hippocampal neurons and finding difficulty in maintaining them for long term. Have gone through several papers and found different way to...

19 October 2022 3,640 0 View

Which is better EDXRF Rigaku NEX CGII or Bruker S2 Puma Series 2 or Shimadzu EDX-8100 For texting of oxides in cement and P in steel bars?

which is better EDXRF Rigaku NEX CGII or Bruker S2 Puma Series 2 or Shimadzu EDX-8100 For texting of oxides in cement and P in steel bars we are about to purchase an XRF also, our P requirement...

19 October 2022 4,749 0 View

Gait analysis software for mac?

I need a software for gait analysis that can run on mac, any help!

14 October 2022 457 8 View

How to analysis FTIR data of newly synthesis nanoparticles?

How to read and understand FTIR data of nanoparticles?

13 October 2022 731 7 View

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