I have tested enzyme activity (b-galactosidase) with cation ions in different buffer and the results differ dramatically. For example, while I used MES buffer calcium acts as an activator but when I dissolved my enzyme in phosphate buffer calcium acts as an inhibitor.
is there any explanation besides I done something wrong?
Kindly ask for help and thank you for any answer.
Hello,
I have a question: have you tested your enzymatic activity with the buffers without adding calcium?
Why am I asking you this question, because I told myself that perhaps it is the buffer itself (MES) and not the calcium which will activate and give optimal conditions to your enzyme.
the pH and ionic strength of the buffer can also impact the enzyme's activity by affecting its conformation and the interactions between the enzyme and its substrate. Therefore, the specific ions and their concentrations in the buffer can have different effects on enzyme activity depending on the specific enzyme and its substrate.
- Badges
- Science topic
- Similar topics
- Agriculture
- Horticulture
- Seeds