Review and cite SECTIONING protocol, troubleshooting and other methodology information | Contact experts in SECTIONING to get answers
4,466 views 756 posts
Questions related to Sectioning
We only seem to be able to download .ENS files in section "Download Data" in Pulse, but we would like to have csv or ASCII.
20 October 2021 1,352 1 View
I do free floating sections for IHC and i would like to eliminate xylene in the process. Is it possible to leave out the clearing in xylene before coverslipping? Or will it have an affect on the...
15 October 2021 8,823 4 View
I am looking for a good RNA isolation method. Most methods I have found take the whole tissue section to make RNA. However, I would like to take a small region of interest from a tissue section to...
12 October 2021 4,760 3 View
As you might imagine the sections are very big. 4X4mm. the trouble is that my glass knife gets dull right away and my 0.5um sections quickly become 1um sections. and when that happens, the...
28 September 2021 7,825 4 View
In FDTD there are two kinds of farfield results, namely antenna gain and scatterer RCS. So what's the difference between positive and negative RCS (in dB)? Is it related with energy absorption?
26 September 2021 7,350 2 View
Salam I am conducting a single point calculation on an optimized structure of chitosan-ZnO nanocomposite with a DFT/B3LYP/LanL2DZ method to get the energy of the anionic form of the ground state....
24 September 2021 4,455 4 View
Hi dear researchers, I hope doing great. Lighting waste represents a significant source of critical metals such as Gallium. But, the procedure that is used to recover Galium(especially in the...
24 September 2021 1,958 0 View
I am doing in situ TUNEL staining on mouse tissue sections. I see a fair number of bright TUNEL-positive, DAPI-negative events in many sections. I have seen in some papers that cells lose DAPI in...
21 September 2021 4,082 3 View
I am interested in comparing fish abundance along the river's continuum (10 sampling sites along a 40 km river longitudinal gradient). Sampling would start in upstream sections, that are shallow...
20 September 2021 4,119 13 View
When writting the results of the thesis do we need to consider only the % of responses represented by a majority for a particular question or is it ok to report % represented by a minority of...
17 September 2021 7,967 7 View
I am currently doing a topology optimization of a given elastic tensor. My cost function is the square of the difference between the target elastic tensor and the elastic tensor, and my volume...
11 September 2021 8,100 1 View
I have the maximum and minimum limit of angle deviation. I have splited the interval into 10 sections. How can I find the probability of deviation as a percentage? Thanks in advance
09 September 2021 4,913 1 View
I'm wondering if i can find any lighting standards for different wards or section of an hospital or not? is there any detailed code for it? Thanks
06 September 2021 9,109 3 View
Cyclic response of infill walls in time history plot option in perform 3d using section cuts is not giving suitable results.
06 September 2021 8,290 2 View
Hello everyone, I want to do IHC on brain free-floating sections. Has anyone tried this before? If yes, what's the best protocol. I usually do IF using these sections, but I want to try IHC....
03 September 2021 8,818 4 View
I am a beginner in Gaussian 16 and trying to learn the BEB method. I finished calculating the optimized structure using DFT (B3LYP/6-31G(d)). I have attached the equation used for BEB calculation,...
27 August 2021 3,959 2 View
I'm studying heat transfer in a commercial heat pipe for solar collector.The heat pipe condenser section is attached to the solar collector manifold, and water at different rates of temperature...
25 August 2021 1,263 8 View
Initially I had more variables as per the framework, however, I realized later after conducting EFA, that some of the variables do have convergent validity which will make the analysis...
24 August 2021 8,056 4 View
Many empirical papers in economics do have a separate section for robustness analysis after the main estimation which might be the use of another estimator or perhaps using new sets of variables....
22 August 2021 8,523 3 View
Protocol for seed sectioning hard ones
18 August 2021 3,526 2 View
Does anybody know a good protocol to fluorescently lable tubulin in cardiac muscle histological sections? The best I got when imaged with a scanning confocal microscope was a fragmented network of...
18 August 2021 2,633 4 View
I am looking to seek suggestions on how to pre-complex primary and conventional secondary or primary and conjugated secondary to use for IF on FFPE sections. Any tips, protocols, suggestions or...
18 August 2021 4,286 7 View
I have H&E stained hippocampus sections and would like to look at thickness and number of degenerated cells of hippocampus at different areas in different studied groups to confirm neuronal...
17 August 2021 6,610 3 View
I have an O shaped water bath with a square cross section that I am circulating with an immersion circulator. How do I measure the flow rate at a given point in the bath? Can I use an anemometer...
16 August 2021 3,740 3 View