Review and cite SECTIONING protocol, troubleshooting and other methodology information | Contact experts in SECTIONING to get answers
4,957 views 1,179 posts
Questions related to Sectioning
I perform free floating immunofluorescence in mice brain tissue. My sections shrinks and form lumps when I transfer them from TBS to blocking solution or sometimes from primary antibody to TBS....
15 January 2022 8,350 1 View
I forgot to soak my rat brain samples in 30% sucrose solution following perfusion and embedding in OCT compound. I am trying to section the brain, however it is quite difficult to get a good...
12 January 2022 6,162 2 View
· In Extrusion process, it is evident that the extruded section was bent due to the material micro-structural and forming interface condition related factors. From application point of view, how...
09 January 2022 4,335 2 View
Dear colleagues, Currently I'm doing research using ARDL-ECM method processed through Eviews 10. Can somebody mention or explain what are the robustness test used for ARDL-ECM analysis? Thank you
01 January 2022 2,821 5 View
Who is interested in co-authoring an article about the role of action research in improving the transition from educational plicy to implemetational spaces?
24 December 2021 1,450 8 View
Hello everyone, We are struggling with fluorescence immunostaining of paraffin-embedded sections of TA muscle. We're staining with laminin, pax7 and eMHC. When we first stained I used the...
21 December 2021 8,205 1 View
New to Abaqus, trying to model a 2D rectangular beam with thickness to conduct buckling analysis. However, I am only able to use Shell feature draw the beam length but can't assign thickness or...
09 December 2021 883 3 View
Cd 48: with Very hight capture cross section but is a toxic metal or Ni 28: also with hight capture cross section but around 8 Mev prompt gamma Gd 64: good but expensive any suggestions ?
09 December 2021 4,329 5 View
I'm using a cross sectional data to study poverty reduction in China, and have a poverty rate variable collected in different years according to case's region. The problematic variable's...
07 December 2021 3,620 6 View
The undulation frequencies of topography can be calculated directly. However, what are the undulation frequencies of the density interfaces inside the crust (e.g. the interface between the upper...
06 December 2021 3,195 0 View
Dear all, I am given some free-floating coronal sections of the marmoset brain to do immunostaining. it's important in my research to identify the left and right sides of the sections, and I have...
02 December 2021 3,022 7 View
The following situation: I have a map (JPG) in which cross sections are drawn and the corresponding cross sections as image files (also JPG). The cross sections have (as it is usually the case)...
02 December 2021 5,560 3 View
Atom index (1135) in position_restraints out of bounds (1-3). This probably means that you have inserted topology section "position_restraints" in a part belonging to a different molecule...
25 November 2021 9,508 0 View
I'm design to culture mouse aorta in vitro and give some cytokines stimulation. After that, I will collect the vessle for parrfin section. I want to search some information for this, but there...
23 November 2021 6,020 6 View
Attached I have a D5000 tapestation from a partially prepared library from a new protocol i'm trying. This is a pooled library prep where cells are lysed a 384-well plate in 1% triton and RT...
23 November 2021 2,464 0 View
Hello, I stained mouse spinal cord sections using Nissl stain (cresyl violet). I am wondering how I can count for neurons, and differentiate between neurons and glia. I have attached an image...
18 November 2021 4,444 3 View
I have a query, I want to do TEM of a Polycaprolactone polymer film (~150-180 micro meter thick). I would like to know what is better wet cryo sectioning or dry cryo sectioning. Can anyone...
18 November 2021 4,589 3 View
I am experienced staining frozen sections on the slide and free floating. These sections required only sucrose protection prior to sectioning. I have been tasked with developing and IHC program...
13 November 2021 6,535 15 View
W, Mo, Re, Os, V, Nb- these are common refractory metals, and I know of volatile oxides of W, Re, Mo and V. What about other metals? If this is the common trend, then why is this so? are the...
12 November 2021 2,018 4 View
This may be a stupid question but I am getting myself confused. I have run the analysis for my mediation model using Hayes PROCESS and in the process of writing the findings up. However, when...
11 November 2021 3,567 1 View
Dear All, I have the MATLAB files of the isolated benchmark building. However, in those files the building is modeled using its eigenvalues and no information is given regarding the columns,...
09 November 2021 8,487 3 View
I am trying to preform an immunohistofluorescence in GFAP-eGFP transgenic mouse cortical brain sections. The protocol involves antigen retrieval with citrate buffer followd with blocking, washing...
09 November 2021 1,978 3 View
Keypoint ORIENTAION Angle or Layup 59 [-65, 5, 5, 0, -65, 85, 90, -30, -30, -30, 60] 67 [5, -65, 0, -65, 90, -90, -50] 71 [-75, -75, 65, 0, 0,...
04 November 2021 9,675 2 View
Good morning.I am a graduate student in the College of Forestry, Guangxi University.I have read the article《Brittleheart as a critical feature for visual strength grading of tropical hardwood:...
02 November 2021 7,272 3 View