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I am trying to purify a his tagged protein using IMAC. The homologs of this protein purify very well. However, this protein precipitates on elution. Buffer composition- 20mM Tris-HCl pH7.4, 500mM...
21 March 2024 9,056 4 View
The answer will assist me in determining which solution to recommend.
20 March 2024 5,683 3 View
I used the CNTFET model Verilog A file from Stanford University to design pcntfet and ncntfet
19 March 2024 7,294 0 View
I have THP-1 cells differentiated using 20ng/ml PMA and incubated in 12-well plates at 2x10^5. I would like to lyse the cells and extract LL-37 or hCAP18 from my cells to use for an human LL-37...
19 March 2024 2,197 4 View
Hi Dear Researchers, Providing some background information, I am an undergraduate student, completely new with QGIS, and I am about to use it in my graduation thesis. On the other hand, I am...
19 March 2024 6,280 2 View
Is a broad concept that balances the need for economic growth with environmental protection and social equity and need to maintain environmental balance?
18 March 2024 181 1 View
Respected researchers, teachers and scholars, Kindly suggest the names of the names of the Free and fast publishing journals > 6.00 NAAS regarding Cropping systems
16 March 2024 4,673 0 View
or provide previous studies and references.
15 March 2024 3,856 0 View
I'm looking for sources of funding for science communication activities. Do you have grants or calls you can recommend? I am from Latin America and work in the fields of biology, entomology, and...
15 March 2024 2,720 1 View
Hi, I am looking for computational tools to get a score/measurement of goodness of fit/binding affinity/any other metric to present a binding between a protein and a peptide, preferably via Pymol....
13 March 2024 8,808 1 View
Additionally, could you suggest technical analytical instruments suitable for this purpose?
12 March 2024 7,530 2 View
Please introduce me to some references and provide formulas. Thanks
12 March 2024 1,798 1 View
I want to cultivate MDK spheroids in non-adhesive microlunks from agarose. I have not found such an approach in the literature. The articles are exclusively about matrigel. I want to know if...
12 March 2024 869 0 View
Hello, I'm studying rainfall and temperature's impact on river basin water quality. Are there any indices that effectively capture both rainfall and temperature? I have only temperature and...
11 March 2024 7,183 3 View
An explanation of major issues faced by analysts while analysing risk in wealth management, investments and portfolio management. Any ways to tackle them or the root causes of those issues would...
09 March 2024 6,845 4 View
Currently working on my master's thesis proposal, which involves exploring the interplay among 7 variables. This has led to the formulation of 13 research hypotheses. I'm curious to hear thoughts...
09 March 2024 4,294 2 View
I want to compute properties of fuel after altering by one hydrocarbon component.
08 March 2024 5,427 0 View
Hi all, I'm looking for any studies that have used or using Design Thinking, in or across longterm projects in organisations. Studies that have implemented DT or measured its impact over a period...
05 March 2024 5,300 7 View
What are the nutritional requirements and nutritional types of bacteria and role of soil microorganisms in plant mineral nutrition?
05 March 2024 1,833 0 View
Purpose of the budgeting and forecasting in an organization especially in the field of managerial accounting.
05 March 2024 9,517 9 View
explain its morphology, taxonomy and habitat distributiion
04 March 2024 1,251 1 View
these sites are running very efficiently and withdrawing money from young researchers. Please take some necessary actions against them if possible. Most of these sites are operating from...
03 March 2024 1,581 2 View
I have decided to use the probe sonication for making a homogeneous MPD solution and g-c3n4 as the nanopaticle to coat for TFN. But whenever I use the Probe sonication, once I start the process, a...
02 March 2024 1,436 1 View
Hi! I'm trying to publish my TFM as a preprint book but I cannot, nor also as a book. Could you help me, please? Thanks Best regards
01 March 2024 2,575 3 View