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DI (Veeco, Bruker) Dimension AFM not communicating with PC?

Hi all. Apparently all hardware is fine (several green LEDs everywhere, on controller, back of acquisition card, etc.), yet there is no DSP recognized by the PC. Drivers are probably requested for...

02 April 2025 3,137 1 View

How to find drought-resistant genes in rose RNA-Seq data as a beginner?

31 March 2025 2,097 4 View

I am currently doing a plasmid DNA stability experiment in HeLa cells and I had a query regarding the same. Can someone share their views on this?

After transfection of the plasmid of interest by Lipofectamine 3000 method, I harvested the cytosol at different time points, viz., 6,12,24,48,72 hours and checked the plasmid copy numbers by...

31 March 2025 4,794 0 View

Interested in ideas on why choosing sustainable development thinking over sustainability thinking in 1987 is the first development thinking blunder?

Interested in ideas on why choosing sustainable development thinking over sustainability thinking in 1987 is the first development thinking blunder since then? You will find some good food for...

31 March 2025 1,054 2 View

How can you make a case for interactive art/media, when they are not beings as such?

There is no being or entity that has ever been identified as art or media, so how can anybody make a case for such a thing to be interactive?

31 March 2025 208 4 View

To what extent do researchers using qualitative case study methodology following the core features of this approach in their research?

I have noticed that in manty research papers where mentioned (qualitative) case study as methodology are not following a minimum requirement of a qualitative case study, particularly regarding the...

30 March 2025 4,166 3 View

Arabidopsis is not growing optimally. Is this some biotic stress or abiotic stress?

I am currently facing problems in growing Arabidopsis at our growth room. Leaves are observed to be shrinked and yellowing in color. Also the growth is restricted and very late bolting is seen....

29 March 2025 890 4 View

How does the context contribute to determining the intent of the speech?

كيف يسهم السياق في تحديد مقصدية الخطاب؟

29 March 2025 720 3 View

PH effect on bioavailability experiments ?

Dear colleagues, Long time no see ... Today I have a question regarding methods to illustrate the bioavailability of peptide-mimic agents in vivo by using mammalian cells instead of other...

28 March 2025 7,338 4 View

Development of an adsorbent for adsorptive removal of heavy metals from water. which adsorbate is best to use for this topic and why?

why

28 March 2025 3,061 3 View

How to dertermined the median split?

Hello, I am writing a meta-analysis article and the reviewer of the journal has said that you should examine the moderating variables in the article and use the median split method. can you guide...

28 March 2025 1,332 4 View

What is the best way to use tringulation?

If data was collected using the explore study by interviews, then the questionnaire was built based on the qualitative data to rate these collecting data using the Likert scale. How to use these...

27 March 2025 7,129 6 View

How many times can a Cas enzyme be freeze-"thawed"?

I haven't found a real answer online. I understand it is always best to minimize freeze-thaw cycles for all compounds ever, but has anyone here developed a max # of freeze thaw cycles for Cas, or...

27 March 2025 2,670 1 View

Is this mycoplasma contamination?

I've been growing a primary cell line for 2 months now and in the last month growth has slowed down significantly and more cells have become senescent. Since subculturing the cells seemed to have...

27 March 2025 9,163 1 View

How can NGOs leverage machine learning and data analytics to enhance decision-making, optimize resource allocation, and improve social impact?

This research investigates how Non-Governmental Organizations (NGOs) can leverage machine learning and data analytics to improve their decision-making processes, optimize resource allocation, and...

27 March 2025 429 2 View

Broken link to co-author profile ?

Hi, I just posted a preprint, co-authored with Ronald Meester. Meester also has a profile on RG: https://www.researchgate.net/profile/Ronald-Meester but the link to it doesn't work, and the...

27 March 2025 2,795 2 View

How To Publish A Research Article ?

How To Publish A Research Article.

26 March 2025 5,629 2 View

When biogas production in flask and water displacement method, why the water coming back to flask and accumulating during night time?

I'm doing biogas production experiment in 1L flask. I follow water displacement method. And this experiment is conducted in normal room temperature. Current average day temp is around 36°C and...

25 March 2025 6,545 2 View

How to determined the electronagativity of double perovskite for counduction band valance band position?

how to determined the electronagativity of double perovskite for counduction band valance band position?

25 March 2025 6,465 1 View

Any one who can help in NMR and Single crystal analysis via collaboration please?

Need help in NMR and Single crystal analysis via collaboration

25 March 2025 6,516 3 View

Which is the optimal approach for introducing an ATG start codon when cloning a truncated domain with mCherry?

I am planning to clone a truncated version of a protein that includes only the extracellular domain, tagged with mCherry. Since the extracellular part lacks an ATG start codon, I am considering...

25 March 2025 8,819 3 View

I am asking about question on dispersion polymerization?

i used the water and PEG400 as a dispersion medium and Am as a monomer and v50 as a initiato and pvpk90 as a stablizer.when i used the water at 80% it turns into the gel i want to get the stable...

24 March 2025 4,020 1 View

How to avoid this Arabidopsis inflorescence problem?

Hi, I want to collect seeds from my Arabidopsis, but this is happening in some of them. Can you please help me to avoid this condition by telling how do you treat your plant after the...

21 March 2025 1,637 4 View

Can someone help me understand these results?

I did ribodepletion and then used Bioanalzyer RNA 6000 pico assay to test for any remaining rRNA. I see very sharp peak at around 100bp but i don't understand if i should be seeing a sharp peak in...

21 March 2025 5,913 1 View

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