What's the best protein Mol Weght cut off columns?

You should perform gel filtration column chromatography to achieve a useful separation of proteins on the basis of molecular weight.

Ultrafiltration membranes are not a good replacement for full-size gel filtration columns. They are intended for concentrating protein or buffer exchange. They can be used to separate proteins that differ greatly in size, but are not very efficient.

Mini gel filtration spin columns do not have sufficient resolution. They are intended for buffer exchange and desalting.

Nikolay Klyashtornyy

When it comes to protein purification based on molecular weight cut-off (MWCO), there are several options available for microcentrifuge tube-based column filtrations. Here are a few commonly used options that may suit your requirements:

Amicon Ultra Centrifugal Filters: These filters are widely used for protein concentration and buffer exchange. They come in various MWCO options, and for your desired range (80-130 kDa), you can consider using Amicon Ultra-4 or Ultra-15 filters with a 100 kDa MWCO. Here is a link to the product: https://www.sigmaaldrich.com/catalog/product/sigma/ufc910096

Vivaspin Turbo Ultrafiltration Units: These units provide rapid and efficient protein concentration. For your target range, you can consider using Vivaspin Turbo 15 units with a 100 kDa MWCO. Here is a link to the product: https://www.sartorius.com/shop/ww/en/usd/ultrafiltration--protein-concentration/c/MC-ultrafiltration

Thermo Scientific Pierce Protein Concentrators: These concentrators are available in different MWCO options and are designed for protein concentration and desalting. For your desired range, you can try the Pierce Protein Concentrators with a 100 kDa MWCO. Here is a link to the product: https://www.thermofisher.com/order/catalog/product/88502

It's worth noting that different manufacturers offer similar products, so you can explore different brands based on availability and your specific preferences. Additionally, it's always recommended to confirm the compatibility of the chosen column filtration system with your sample and experimental needs before purchasing.

Please note that while these products are commonly used for protein concentration and desalting, they may not remove all proteins below the specified MWCO. Some smaller proteins or contaminants may still pass through the filter to some extent. Therefore, it's essential to consider other purification methods or techniques if complete removal of lower molecular weight proteins is critical for your application.

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