I am looking for a IoT simulator ?

I am working on a university project so, I m looking for a good Simulator compatible with LoRa and LoRaWAN protocol to simulate an IoT scenario. Thanks in Advance

20 February 2021 6,352 6 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

Which is the best silane for making a borosilicate glass surface hydrophobic permanently? and what is the protocol?

Regarding my needs I am looking for a glass coating agent that makes the glass hydrophobic permanently

09 February 2021 3,924 5 View

How can I set up UV irradiation for crosslinking lab?

Hi, I am using a mix of 2 polymers and want to try and crosslink them. I have a UVA lamp of 365nm and have tried the lab previously at 30cm above. How would you recommend I approach the lab and...

31 January 2021 8,711 3 View

Which air pollution/air quality books do you recommend for working with environmental engineering students?

I am exploring up-to-date books on air pollution and air quality, and I would like to know which books do you recommend for environmental engineering students.

25 January 2021 3,972 7 View

How to simulate Sloshing water using Dynamic mesh in ANSYS Fluent ?

I am trying to model sloshing water in a tank in ANSYS Fluent using Wall motion. BUT it seems that System coupling is not responding to wall motion and it works only with Dynamic mesh.

20 January 2021 2,963 3 View

Are there other models that meet the same purpose?

Both Jakes and Gaussian models are used to characterize the power spectral densities of mobile communication channels.

11 January 2021 759 3 View

What is the Role of temperature In Hummers method for the preparation of graphene oxide?

04 January 2021 5,033 2 View

SDAS, secondary dendritic arms space relation with solidification rate?

Want to know the relation between sdas and solidification rate, the mechanism by which getting smaller space between secondary arms by increasing the rate ?

23 December 2020 735 2 View

Any recommendations for a good CRISPR/Cas9 commercial system for functional genomics of fungi/yeast?

I look forward to implying a good CRISPR/Cas9 system with a selective maker for functional genomics of important genes from environmental fungi and yeast.

22 December 2020 1,432 2 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How to revive a Hyphomicrobium strain?

11 February 2021 3,524 2 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

How to measure volume of Pinus roxburghii ?

I want to measure volume of Pinus roxburghii in the Jammu region of India to calculate the carbon stocks of Pinus roxburghii.

31 January 2021 2,311 3 View

Where can I find the information about steel final production in Japan?

I am doing research about the in-use stock of iron and steel in Japan. I need the report about annual yield of steel final production. I found some information in the webpage of Japan Iron and...

25 January 2021 3,867 2 View

How to select substrate concentration to determine the inhibitor behavior as competitive or non-competitive?

I already calculated the IC50 value of the inhibitor against the protease enzyme. Now I need to find this inhibitor competitive non-competitive. I decided to perform the Lineweaver Burk plot, but...

21 January 2021 8,378 3 View

How to revive bacteria from glycerol stock?

19 January 2021 7,025 4 View

How can I prepare the erythromycin stock?

I need to prepare a stock of 10 mg / ml erythromycin to check the mic value. I am trying to dissolve 0.1g/10 ml of water but i can't dissolve erythromycin in water. How can i prepare 10 mg/ml...

03 January 2021 9,947 3 View