Plant Extracts

Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away,... | Contact experts in Plant Extracts to get answers

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Questions related to Plant Extracts

Apoptosis , Stress oxidant, MDA variation ?

I am studying the influence of doses of plant extracts on rats, since the plant is toxic, the value of MDA (oxidative stress) increases with increasing doses. the problem is that for the maximum...

30 August 2021 4,012 4 View

Why did UV-Vis spectrophotometer results show multiple wavelengths?

I did a UV-Vis Spectrophotometry on a sample of plant extract and silver nanoparticle between 200nm to 800nm, but the results showed multiple signals and wavelengths. What could have gone wrong?

28 August 2021 6,324 3 View

How can we relate MFI values with Concentration? Can MFI results (Qualitative Assay) be substitute of Concentration (Quantitative)?

If there is no Positive or Negative control reference range in terms of concentration, is it ok to use MFI Values to compare the results.

15 August 2021 7,105 0 View

How do I determine the concentration of plant extract after diluting from the stock solution of different concentrations of DMSO?

i prepared 100 mg/ml of stock solution using 99.9% DMSO. When, I found 99.9% DMSO killed the test bacteria in the broth macrodilution, I diluted the 99.9% DMSO to 2%, and found safe for the growth...

26 July 2021 667 5 View

How to quantify terpenoids in plants?

Plants produce a large number of terpenoids for biological signals or against adverse environment. In order to study these substances, I want to know how to determine the content of a terpene in...

26 July 2021 5,326 6 View

How to prepare the dose from a methanolic plant extract?

I have used methanolic plant extract to assess its biological activity. Would anyone suggest how do I prepare the dose, or in which solvent it should be dissolved?

25 July 2021 4,805 4 View

I have a lot of medicinal plant extracts (meroterpenoids, diterpenoid alkaloids, triterpenes), please suggest me some quick activity screening tests?

My job is to extract, separate, purify and identify phytochemicals, I need lots of cooperators to find out their potential bioactivities.

22 July 2021 6,398 6 View

Is it possible to extract antibacterial metabolites from endophytic fungi without using rotary evaporator?

im currently in the phase of extracting metabolites, specifically for antibacterial screening from endophytic fungi. i tried ethyl acetate, but after 1 week it's still not dry.

21 July 2021 6,244 5 View

What solvent and method do I use best to extract both PAHs and Metals from road runoff to be further tested in bioassays?

Hello, I recently started working with road stormwater runoff and want to analyse the ecotoxicological potential of the leachate and the dissolved particles in a bioassay battery. While testing...

12 July 2021 2,670 4 View

How to run an absolute quantification on steroid hormones (e.g. estradiol, testosterone) in human saliva samples using mass spec?

I am interested in using mass spec to quantify steroid hormones in human saliva samples, but I am completely new to mass spec. Unfortunately, there seem to be very few protocols for this, and...

07 July 2021 7,211 0 View

Ethanolic extracts of berries remain viscous, how can I get dry extracts?

I have done ethanolic extracts of four kind of berries 1:10. I concentrated the extracts with the rotary evaporator and transferred the liquids in vials which I left under cap to dry at room...

29 June 2021 9,072 7 View

How to remove the excessive plant extract when we do green synthesis of rGO and metal oxides?

I am preparing nano composite and metal oxides. Plant extract I'm using is so dark that my product gets the color. I'm seeking that how to get rid of that.

28 June 2021 2,194 3 View

Why higher mortality of Zebrafish in treatments with concentration in between?

I currently conduct a toxicity test using zebrafish adults for my methanolic plant extract. I have 100% and 71%cumulative mortality for the fish at the concentrations of 250mg/L and 125mg/L, but...

27 June 2021 4,257 5 View

After chromatographic separation, does it need to evaporate mobile phase in order carryout bioassay and NMR?

I going to check the bioactivities of plant extracts

21 June 2021 9,664 9 View

How does the Vorticella spp. colony count?

Vorticella spp. are the species of protozoa (cilliate) exist in rivers as well as reservoirs , often attache at stalks in a patch. well, is it count by individual or path? (How many is the patch?)

20 June 2021 4,208 2 View

Hi, Can you suggest a suitable spectroscopic technique to elucidate structure of metabolite molecule?

I want to determine the structure of extracellular metabolite of plant extract

15 June 2021 1,741 18 View

Can you suggest a method to isolate compounds from plant extract and to determine their structure ?

I have a plant extract and I want to isolate metabolites present in it. Which chromatographic method and spectroscopy will be ideal for this

15 June 2021 8,250 18 View

How can I prepare a stock solution of a crude plant extract?

Using water as my vehicle, In what ml of water do I constitute 80g of a crude plant extract? I'm working on the Antiulcerogenic effects of the plant on rats. Thank you.

15 June 2021 8,552 6 View

Can I use 99.5% ethanol as solvent to dilute my plant extract for an insect bioassay ?

I have done crude extraction of citronella, lemongrass, garlic and onion using 99.5% Ethanol. Now I want to dissolve the extracts in a suitable solvent to test them against whiteflies. (a) Will...

13 June 2021 566 14 View

Bacterial cell wall and membranes became thicker and hardly visible after exposure towards plant extract?

I introduced plant extracts during conjugation experiment and when viewed under electron microscopes, the cell wall and membrane became thicker and the lines became hardly visible. Any suggestions...

12 June 2021 1,544 6 View

How can I dissolve the non polar fractions of plant extracts to perform antioxidant assays ?

I am working with plant extracts. I need to purify by solvent fractionation and perform antioxidant assays like DPPH ABTS. I am not able to make a clear solution of those non polar fractions....

26 May 2021 5,721 5 View

Should plant extract be in paste or powder form to do wound patch?

Dear Researchers, I need your opinion on, in order I want to do wound patch from plant extract, should the extract must be in powder or paste form? I had already dried the leaves, grind using...

24 May 2021 8,288 3 View

I am looking for any reference related to gold Nano particle green synthesis using weight concentration (m/v) as one of optimization parameters?

It is hard to find any reference related with gold nanoparticle synthesis using plant extracts that were extracted using ethanol solvent and has weight concentration (m/v) as one of its optimizing...

23 May 2021 1,033 3 View

How will I use sephadex for separate plant extract? And which buffer use with sephadex?

Dear researchers, I will work on plant extract. I will seperate the plant extract with sephadex. Could I get information about this method? How will I use the sephadex and which buffer with...

21 May 2021 2,018 2 View

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