Plant Extracts

Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away,... | Contact experts in Plant Extracts to get answers

1,411 views 2,564 posts

Questions related to Plant Extracts

With regards to Western blotting, Why would some of my samples have signal (using AKT & P-AKT antibodies)?

After the transfer and Incubation periods I exposed my membranes (PVDF) on X-Ray Film using Chemiluminescence. After developing the film only some of the samples had bands/signal. What could it...

17 January 2023 171 0 View

Is there a commonly used in vitro bioassay to measure the bioactivity of EPO?

Hi, I want to measure the bioactivity of epo with a bioassay. I am thinking of using TF1 (a human erythroleukemia cell line) cells, but do I use CCK8 (faster, non cytotoxic) or MTT (more...

16 January 2023 5,282 1 View

How to change value of Angstrom (A) in AutoDock Tools?

I am doing docking study on my synthesized compounds and may I know how to change the value of Angstrom? I am using AutoDock Tools for the Grid Box on the Angstrom (A) value. I wished to change...

16 January 2023 715 2 View

Which software is good for processing time lapse study/ data?

Hello I need a software help me to process my time lapse study thank you

13 January 2023 7,362 1 View

How can we prepare odorless colorless polyphenols rich fraction from industrial herbal waste?

with special reference of Nicotine waste.

12 January 2023 4,255 3 View

Making standard curves of Caffeic and Gallic Acid using Spectrophotometry?

Hello there, I'm having problems in making the standard curves for caffeic and gallic acid. I mixed 0.01g (10mg) of each chemicals into 10ml of 90% MeOH solution, so I'd have 1mg/1ml as a stock...

11 January 2023 9,733 5 View

For plant/ Fruit extract preparation protocol , stock preparation methodology how to calculate it?

10gm of plant/ fruit powder was added to 100ml of solvent and subsequently incubated in rotary shaker followed by centrifugation and rotary evaporator evaporation. Afterwards 40ml residue/...

10 January 2023 1,272 0 View

The absorbance value of validation criteria for an ELISA kit refers to raw absorbance value (450nm) or adjusted absorbance value (450nm-620nm)?

Is the absorbance value of validation criteria for a commercial ELISA kit referring to raw absorbance value (450nm) or adjusted absorbance value (450nm-620nm)?

10 January 2023 2,993 1 View

Multimodal and multiorgan domain adaptation-based segmentation?

Looking for papers on generalized domain adaptation-based segmentation, specifically for the following scenario: Dataset A has images of organ_1 in modality_1 (e.g. Cardiac MRI data). Dataset B...

10 January 2023 5,365 0 View

What are the differences between Spuway and CAT+ extraction of bioavailable nutrients?

What are the differences between Spurway and CAT+ extraction of bioavailable nutrients, aside from the extraction agent (acetic acid and calcium chloride respectively)?

03 January 2023 7,955 3 View

How to I combine 2 ligands to 1 receptor for molecular docking using autodock vina ?

I am doing my thesis for the last season of college and ı need to dock to ligand and protein for better binding affinity ı decided to combine the two ligand for moluecular docking so how can I...

29 December 2022 7,222 4 View

How do I calculate the protein content from absorbance and standard curve and express as g/100g or percentage?

I did bradford assay and I want to calculate the amount of soluble protein in my glycoprotein sample. I prepared BSA stock of 1mg/ml and did a standard curve ranging from 0.01 to 0.16 mg/ml. I...

07 December 2022 560 0 View

Is it possible to obtain a patent in 23 days?

One of my known claims to have been granted a German Patent in 23 days. Is it really possible to obtain a German Patent in such short interval of time?

05 December 2022 6,529 6 View

Is there any standard protocol available of calcium green staining in rice leaves?

I need the calcium green staining procedure in rice plants to visualize the calcium dynamics. Is there anyone to solve my query? thank you

29 November 2022 5,110 0 View

How to take 1.93 milliMolar from 58 milliMolar stock solution ?

Has per our reference we made 58 mM stock of reagent metol and for working we have to take 1.93 mM from stock, what is the method to take working or any dilution factor or formula available ?

24 November 2022 3,650 0 View

How to calculate the degree of crystallinity for Polypropylene with multiple melting peaks?

I've been trying to study the degree of crystallinity of Polypropylene using DSC. In the heating curve I find two melting peaks - one at around 150 and the other at 160 degree Celsius. I think...

23 November 2022 5,864 0 View

Which the best software to simulate a SEM images for characterization of thermal spray coating ?

I did an SEM test on thermal spray samples and need to simulate the SEM images to characterize the coating.

17 November 2022 7,139 0 View

extracting phenolic compounds from medicinal herbs for estimation by HPLC?

The simplest methods for extracting phenolic compounds from medicinal herbs for estimation by HPLC With the least organic solvents and in an economical and inexpensive way

15 November 2022 1,536 3 View

How to make final 3 ml volume with the given three agents ? volume of each reagent ?

A test was carried out in a final 3 ml reagent mixture containing 1.93 mM Metol, 68.6 lM copper and 1 mM acetic acid/sodium acetate buffer of pH 5.4.

13 November 2022 8,210 9 View

When assembling Novaseq and Hiseq ddRADseq runs, my Hiseq runs have large amounts of missing data, is there an incompatibility between the two?

I'm using the Novaseq 6000 and HiSeq 4000. Assembled alone, the Hiseq data has little missing data and many reads per sample, Novaseq has more missing data but still is useable. When I assemble...

13 November 2022 4,626 1 View

How to remove clumping of nucleus from mouse skeletal muscle?

We are trying to isolate nuclei from mouse skeletal muscle. After the isolation process, the clumping of nuclei was still present in the slide. This type of clumping nuclei is not acceptable for...

08 November 2022 9,359 0 View

Which active component in a green tea act has highest anti-inflamatory effect?

green tea and its benefits in osteoarthritis condition.

06 November 2022 6,792 2 View

Have anyone of you done electrochemically reduction of CO2?

In electrochemical reduction of CO2 what is the purpose of the following method? 1. Linear sweep voltammetry 2. Cyclic voltammetry 3. Chronoamperometry

03 November 2022 4,673 12 View

How to reduce BOD of an effluent which has a COD between 1700-2000 ppm and TSS below 100 ppm??pH of sample is 7.??

I have tried dosing H2O2 but did not get any positive results. I have also tried Chemical Precipitation using coagulants but I have not reached the benchmark. The COD of Effluent is in the range...

03 November 2022 5,552 19 View

Badges
Science topic