Experimental Pharmacology textbooks?

More André Antunes's questions See All

How can upload an article research gate ?

I want to upload my articles drafts

29 June 2024 2,016 3 View

How can a single photon propagate in a straight-line in a dielectric medium?

Given the well-documented quantum Rayleigh scattering of single photons in a dielectric medium [1] A. P. Vinogradov, V. Y. Shishkov, I. V. Doronin, E. S. Andrianov, A. A. Pukhov, and A. A....

17 June 2024 7,692 9 View

Which is the best model for hypoxia in cell culture ( MSC or fibroblasts)?

I am tring to culture cells in hypoxia using Cobalt chloride model , but I want to compare with antoher method. Our research is about circRNA expression - qPCR and nanopore oxford sequencing.

05 June 2024 2,246 1 View

Why do you name scientists just A. Silva, as there are multiple scientists with the name Silva whose name starts with an A. ?

You sended me a couple of guestions "Did A. Silva author this work". I Looked at the article and found that just the University of Lisbon has over 10 scientist with that name.

13 May 2024 3,524 0 View

ARMAX model to Transfer Function?

Hi i have estimated an armax model using python sippy library. The estimation gives me two transfer functions H and G. How can I combine them into a single one to predict model output for new...

20 January 2024 1,221 2 View

Parasite Eggs Identification, can anyone identify this eggs?

Hello, I'm working on my MSc project related to Parasitology of Marine mammals, particularly cetaceans. I've been identifying some eggs and adult specimens by morphological methodologies, however,...

23 December 2023 2,042 1 View

Foundation models as knowledge-based systems?

Hello, during the recent emergence of LLMs and foundation models I restarted thinking about the very early machine learning attempts to incorporate human expertise and knowledge into expert...

22 November 2023 5,625 0 View

Why won’t PEG stay neutralized?

Upon receiving PEG we titrate to find its base value so it can be neutralized with HCl for certain processes. It’s typically basic on the micromolar level (I think from residual KOH or another...

10 November 2023 6,960 1 View

How to clean Titanium Foil as current collector for batteries?

Hi anyone, I am starting using Ti foil for zinc-ion battery current collector, but i want to know how to clean the Ti Foil. Thank you

25 October 2023 3,649 4 View

O que você acha da Ilha de Lixo Plástico que boia pelo Pacífico, matando animais marinhos que se alimentam deste lixo?

Nanotoxidade Ilha de Lixo no Oceano Pacífico Mortalidade de animais marinhos

22 October 2023 2,685 19 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

Hiralal Merwade

A Hand Book on Experimental Pharmacology by Martin C Michal by Springer publication

André Antunes

Hiralal Merwade Dear Dr. Hiralal these books cover specific methods for different organ systems, correct?

Ovarall view of the procedures can be understood by referring this book

Good Research Practice in Non-Clinical Pharmacology and Biomedicine?