How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Is there a way to get rid of TEA in a TLC plate in order to do proper staining?

Dear colleagues: I have been trying to establish a few HP-TLC methods in the lab. In general, for quantification purposes, I prefer CuSO4+acid charring, to primuline staining. Lately I have been...

20 January 2021 9,019 1 View

What does this ghost peak come from?

We analyse oligonucletide using Ion-pair reversed phase chromatography. The mobile phase A is composed of triethylamine (TEA), hexafluoroisopropanol (HFIP), water. And the mobile phase B is the...

27 September 2020 4,210 3 View

How can I remove triethylamine tfa salt from my peptide?

I'm now purifying a modified tripeptide which is quiet non-polar and sensitive to acidic condition. Thus I added triethylamine (TEA) to neutralize the HPLC fraction which contains 0.1% TFA and...

07 August 2020 5,396 3 View

Total of polyphenols in tea extracts conc mg/l to mg/g dry tea?

04 March 2020 3,649 7 View

Whether DMAP can be used to acylate primary alcohol group in cellulose with succinic anhydride???

I want to succinylate primary alcohol group in cellulose with succinic anhydride by using DMAP as catalyst. is it necessary to keep ph of reaction mixture acidic during succinylation??? can we...

09 October 2019 1,902 1 View

How to calculate the percent yield of BODIPY?

08 September 2019 199 4 View

Fuel cell alternative membrane materials?

Hello, I am working on several novel fuel cell types catalyzed by organic-anorganic composite catalysts and I am grateful that I was given a A4 sized piece of membrane made from quaternized...

07 August 2019 6,879 8 View

TBMDS Protection of a Phenol going way over expected time?

Hi - first post here please let me know if there's a format I ought to follow. I'm running a TBMDS (TBS) protection of a phenol molecule that was previously purified by flash chromatography; no...

05 June 2019 7,264 5 View

Need information regarding synthesis of N Succinyl Glucosamine?

I have been trying to synthesise it using Glucosamine HCl and Succinic Anhydride along with triethylamine. But I am unable to get a good yield of the product. Kindly provide valuable suggestions

03 April 2019 5,898 5 View

The reaction condition of amide and 2,2'-dipicolylamine?

Excuse me, have you ever done a similar reaction as shown in the figure? 2,2'-dipicolylamine is reacted with an amide. I have tried using potassium carbonate, triethylamine, DIPEA as an acid...

02 March 2019 3,975 2 View