When conducting an MTT assay to evaluate synergism between two drugs, do I add the same amount of the drugs as in their combination?

02 October 2022 1 9K Report

For example, if I am adding a 100uL of each drug in the single treatment wells, do I add 200 uL of them combined in the co-treatment wells or just add 100 of their combination?

Guillaume Forget

You have to compare the quantity of drug.

Therefore, you can use 100 µL of the combination (50 µL of drug A and 50µL of drug B)

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