Any one please explain the procedure of how to calculate PPO and POD enzyme activity ?

17 August 2022 0 5K Report

after taking the absorbance within 30 sec of interval upto 3 min

Badges
Science topic

Can I use triphenyl tetrazolium chloride (TTC) instead of nitro blue tetrazolium (NBT) for the estimation of SOD activity?

Both TTC and NBT yield formazan when interacting with oxides. The superoxide dismutase assay requires NBT, which reacts with oxide to form formazan. TTC also produces Formazan when it interacts...

12 February 2024 7,778 0 View

Some basic questions about Michaelis-Menten- is there a conflict in the substrate affinity and catalytic kinetics?

Dear Seniors, Recently I am leading a work on nanozymes and tested the steady state kinetic curves corresponding to the color development reactions for TMB substrate and H2O2 substrate. We...

28 May 2023 5,714 0 View

How to use POD and DMD to determine coherent structures of airfoil flow for one timestep/snapshot?

Please, I want to use POD (Proper Orthogonal Decomposition) and DMD (dynamic mode decomposition) to compute the modes of fluid flow over an airfoil only at one timestep/snapshot (for example,...

12 February 2023 4,758 3 View

How to consider carrier wave frequency for implementing PD,POD and APOD modulation techniques ?

what is the procedure to know how much frequency required for carrier waves and amplitude of carrier waves ?

26 October 2022 9,815 1 View

How to convert switching table of multi-level Inverter into Boolean Expressions for generating firing signals in PD,POD,APOD Modulation Techniques?

Every multi-level inverter has its switching table. For implementing PD,POD,APOD Modulation Technique we have to give correct switching pulses ,for this we need to use logic gates. How is the...

15 September 2022 3,647 0 View

I need native-PAGE troble shooting about band shape?

"Hello! My friend. Stay awhile and listen!" You may able to save soul of a poor graduate student. I have trouble native-PAGE. Here's my test condition. Rice soluble protein, extracted by...

10 November 2021 9,287 4 View

I want to perform Antioxidant assay for my cyanobacterial sample ,can any body suggest complete protocol for the assay like POD,SOD,CAT,APX?

please suggest suitable extraction buffer for preparing cell free enzymatic extract of cyanobacteria. NOTE-we have some protocol which uses 1. 50mM KPP 2.1mM EDTA 3.1% PVP 4.0.5%TritonX 5.2.5mM...

14 July 2021 9,149 4 View

Could anyone explain me the GOD POD method,in which situation we can used it?

how to perform GOD POD METHOD FOR THE ESTIMATION OF GLUCOSE

14 July 2021 8,191 1 View

What is the procedure and method for catalase and peroxidase (POD) activities?

Greetings, I have culture filtrate and cells of bacteria and I'm going to do catalase and peroxidase activities for them (Antioxidant activities). Can any body tell me what is the reagents used...

18 April 2021 1,303 4 View

Why is the confirmatory model (CFA) with master latents unidentified?

I wanted to perform a confirmatory analysis on Schwartz PVQ questionnaire part. The construction of the questionnaire assumes that individual items combine into dimensions (latent variables),...

14 January 2021 2,179 9 View