I am trying to determine the band location and size for a knockout mouse. This would be utilizing the cre/lox method. I am breeding both a fl/fl and cdh5-cre-ert2.
Estos articulos deben tener estas caracteristicas resumir los siguientes aspectos metodológicos: tipo de enfoque de investigación (cuantitativo, cualitativo o mixto), objetivo, hipótesis, muestra...
15 May 2024 820 0 View
The question is pretty straightforward, I think. I was just searching for empirical research (econometric modelling, specifically) on segmented labor markets. I didn't found much, maybe because is...
21 April 2024 3,344 2 View
This question is written in relation to the article written by Neuwinger, Nick et al. entitled “Underfilling of vacuum blood collection tubes leads to increased lactate dehydrogenase activity in...
12 April 2024 371 0 View
This question is asked in relation to the article, "Red and white blood cell morphology characterization and hands-on time analysis by the digital cell imaging analyzer DI-60". The mentioned study...
11 April 2024 4,094 1 View
Dear all, I am trying to build a Brillouin microscope and I was wondering what the requirements are for the laser. Specially in terms of linewidth, stability and tunneability. Thanks beforehand.
01 February 2024 4,808 0 View
Dear all: I would like to perform a post hoc test with R for two independent variables, as well as their interaction. Have someone did it before? I would appreciate any advice. Thanks beforehand
29 January 2024 3,736 6 View
Hello, I greatly appreciate your help in my following statistic task. I need to reduce the number of financial ratios (FRs) - currently 20 - that best represent the firms' profitability in the...
07 January 2024 8,024 4 View
Is is possible that Cretaceous fish, notosuchians, lepidosaurs, or other vertebrates have a morphology that might be confused with "spoon-shaped" sauropod teeth? I have seen a fossil isolated...
08 December 2023 7,040 2 View
During my immunohistochemistry procedure, after applying the chromogen diaminobenzidine, I mistakenly added other reagents—PBS, Triton, avidin, and biotin—resulting in chromogen precipitation and...
30 November 2023 6,702 1 View
I'm looking to explore the relationship between coding schools in a community / region, and regional economic development in technology related fields. Are companies in a community or region who...
22 July 2023 8,436 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View