Does E. coli lack Non homologous end joining method for DNA repair
I am working on Exosomes that is derived from cells that are engineered to express scFv of Anti-HER2 antibody. I want to see its expression in TEM. Can I directly label the exosomes with the...
25 April 2024 9,777 1 View
Can somebody share a standardised protocol for isolating phages against gram-positive bacteria
05 January 2024 8,021 1 View
Can anyone share the protocol for preparing synthetic urine?
23 October 2023 3,352 1 View
Phage enriched in SM buffer and 10x enrichment bacteriophage broth produced crystals upon storage. Can any one tell me the reason for this?
13 June 2023 7,538 4 View
Is there any specific protocol for isolating phages against gram positive bacteria?
07 May 2023 1,769 2 View
Is there any media or molecular method by which we can distinguish Pseudomonas putida from other Pseudomonas species
04 March 2023 4,557 1 View
I have prepared a standard curve for Pyruvate for ALT and AST estimation in serum using manual method. I need to convert Pyruvate (uM) to transaminase activity in U/L. I referred to literature and...
20 May 2021 8,374 4 View
I did experiment on dye degradation using pure and doped TiO2. Both turned the solution colurless after photocatalysis. Is it possible for pure TiO2 to degrade the dye?
18 October 2016 4,356 3 View
I have been researching on different frameworks of (GRC) Governance, Risk and Compliance Management .. and want to implement those framework concepts into a tool using nodejs and R-Land. Please...
26 August 2016 6,994 6 View
Till now Traditional N-Gram approach is used for Word Prediction system.
09 December 2015 7,649 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...
23 July 2024 3,490 6 View
