Hi everyone, I'm at the moment trying to analyze mRNA-seq results from Rattus norvergicus. As you know, there is a lot of options to do GO analysis, not only https://geneontology.org/docs/go-enrichment-analysis/ but also other options like Enrichr, ShinyGO and so on, websites were you could consult simultaneously Gene Ontology, Pathways, GSEA...

My question is what is the better option? considering that for example, using Enrichr you are not able to select your specie but founder indicate that human, mouse and rat databases are included... Is it better then to use GO or Shiny where you are able to restrict to your specie? also, better to give your gene list background? or s enough to use the reference genome?

Thanks a lot.