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Questions related to Nucleic Acid Isolation
Hi everyone, I am having trouble extracting total RNA from gram-negative bacteria (non-typeable Haemophilus influenzae) for RNA-seq. I am following good laboratory practices such as working in a...
07 December 2016 5,165 2 View
If we do not use a spin column protocol, can we get better yield of FFPE DNA using a conventional method?
24 October 2016 5,685 2 View
How can I concentrate extracted nuclic acid after extraction?
23 March 2016 577 5 View
Previously, I try to isolate DNA of Neisseria gonorrhoeae by DNA isolation kit but it's quite hard because colonies of it can't be suspend easily in buffer. With vortex and scrape, it's still...
15 November 2015 5,758 3 View
I would like to use DNase I (from bovine pancreas, Roche) to get rid of DNA. Roche/Sigma does some recommendations (1 mg/ml enzyme, pH 7.0, Ca 0.12 mM, some Mg), but I did not find a comprehensive...
07 September 2015 10,058 4 View
Does the results effect if i could not involve phenol extraction step?
19 August 2015 4,756 2 View
Hi everyone, I'm now working on DNA and RNA extraction for FFPE samples using "RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE" (Life Technologies). After digestion by Proteinase K (usually...
01 July 2015 3,414 6 View
Hi, all, I am looking for a similar product for replacing CHROMA SPIN Columns. It would be great if it would be able to remove DNA size smaller than 800 or 1000bp. Is there any suggestion? Thanks...
15 June 2015 6,357 3 View
I want to prepare solutions with nuclease free water to run RNA samples(isolated from Mirvana isolation kit) on denaturing acrylamide gel. It is given in protocol not to use DEPC treated TBE. So...
30 March 2015 4,864 10 View
Dear All I want to calculate the Tm value of Salmon Sperm DNA, that I purchased from Sigma (31149-10G). The DNA come in powder form, that I was dissolve and purified by standard protocol. The Abs...
24 February 2015 6,202 5 View
anyone who knows a convenient method for fungal DNA extraction that does not need to use liquid nitrogen or glass beads?
27 January 2015 8,661 4 View
Our experiment need large quantity of supercoiled plasmids, we have already tried a lot of methods. Your input will be welcome.
11 December 2014 7,676 5 View
I'm trying to get in vitro transcribed RNA from a linearized plasmid, using the T7 MEGAscript kit. After the reaction I run a few ul on a 1% gel to check my RNA, but sometimes I get no RNA product...
11 December 2014 7,132 3 View
What is the best way to keep the DNA active for long time?
29 November 2014 6,303 3 View
neuroscience
28 November 2014 2,608 3 View
I've tried Qiagen RNeasy and Trizol but the yield and quality (RIN ~2.8) is quite poor. The cards are spotted with fresh blood and left for 2 hours to dry at room temperature. I perform extraction...
20 November 2014 1,318 3 View
Hi, currently I am working to isolate DNA for 16s RNA sequencing (metagenomic purpose) from Beaver guts. I isolate with SDS-based DNA extraction method (Zhou et al. 1996. DNA Recovery from Soils...
17 November 2014 7,533 12 View
I'm extracting from very small amount of tissue using Tri-reagent and I get a low 260/230 each time. Does anyone have any advice?
13 November 2014 5,525 3 View
Hi everyone, I have some trouble in my ChIP experiments. I want to study the recruitment of transcription factor and DNMTs on DNA. For this, I cross-link cell with 1% final of formaldehyde during...
12 November 2014 2,217 4 View
We need to extract pathogen DNA from faeces. However, the amount of the pathogen's DNA (a parasite) is likely to be very small. So, in order to avoid false negative results, we should consider a...
12 November 2014 3,268 13 View
If I want to extract large plasmids from bacteria (more than 100 kb), what is the best way and best kit to get them in pure and high yield. Please help me urgently if you can Best Regards
11 November 2014 8,463 15 View
I am using 1/10 volume of 3M sodium acetate, pH 5.2, for DNA precipitation. However, this salt is so high that it's making problems in electroporation (input = 1500V and 5ms; output = 680V and 0.8...
05 November 2014 8,189 2 View
I have a problem to isolate high-quality RNA from the secondary needles of Norway spruce (Picea abies) for real-time qPCR. I used InviTrap Spin Plant RNA Mini Kit, it works perfectly for RNA...
03 November 2014 7,635 2 View
Hello, friends I will need to prepare some total RNA from cells, however I have never did it before. Everyone says that I need to do it in an "RNAse free zone". What that should be in your...
03 November 2014 1,063 3 View