Nucleic Acid Isolation

Why do I get a pink pellet during RNA extraction after isopropanol precipitation and centrifugation?

I'm doing RNA extraction from plant cell suspensions, using TRIzol protocol. In the last steps, after the isopropanol precipitation and centrifugation, I get a pink pellet. The color only appears...

16 October 2014 7,499 6 View

Removal of mitochondrial DNA from total cellular DNA - can anyone help?

Hi, I am using a technique in C2C12 muscle cells called DamID to tag DNA in close proximity to the nuclear envelope in undifferentiated myoblasts and differentiated myotubes. However, following...

01 September 2014 4,583 3 View

Is 1ml Trizol reagent enough for RNA extraction from 0.5ml blood sample? Instead of Trizol LS?

I have very less amount of blood samples of few avian species and I did not have Trizol LS at the time of sampling so I used Trizol reagent but I read in literature that for blood you have to use...

01 January 1970 7,418 3 View

Which is a better carrier for DNA, RNA isolation from Blood/FFPE samples.?

I wish to use carriers like glycogen, tRNA, Salmon Sperm DNA for improving the yield and purity of DNA and RNA isolations from clinical blood and FFPE tissue samples. Would like to know which...

01 January 1970 9,869 2 View

Precipitation of DNA ?

For the precipitation of DNA during extraction protocol, which is preferred, among isopropanol and isoamylalcohol and what is the underlying reason to select it ?

01 January 1970 5,199 3 View

No title

01 January 1970 891 0 View