Hello, friends
I will need to prepare some total RNA from cells, however I have never did it before. Everyone says that I need to do it in an "RNAse free zone". What that should be in your opinion? Thank you
In my experience this generally means working in a dedicated lab area where you make sure there are as little contamination as possible (DNA, bacteria etc). And all equipment and lab space is regularly doused with RNAse-away spray. Use dedicated pipette tips in boxes that haven't been opened elsewhere etc. Even better if you have a laminar flow hood dedicated to RNA work only, but one have to use what's available, and with some caution, you can make this work even with sub optimal infrastructure
Thanks, Vegard. Do you think I can use regular hood where we work with cell cultures? Because unfortunately it looks like the cleanest place in our lab... :( At least always wiped with alcohol etc.. Or, due to cell work it might be no a good idea?
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