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Questions related to Nanomaterials
Hello everyone — I have locus-specific V3–V4 primers with Illumina overhangs (sequences below) and access to an iSeq 100 (2×150 bp). Before I proceed, I’d appreciate short practical advice: Is it...
20 August 2025 2,373 2 View
Hi, I am using free radical polymerization of MMA monomers (MW=100 Da)in order to produce PMMA. But when I do DSC analysis to find out the Melting point . It simply does not show any sharp peak...
16 June 2025 1,482 6 View
Hello, I've got back some oxford nanopore sequencing (of which I am no expert in), and am slightly unsure as to how to interpret one of the clonal traces. It has this black line that isn't called...
26 March 2025 284 1 View
I have isolated phage against Y. ruckeri. phages titre 108 pfu/ml on double layer agar, while 101 and 102 pfu/ml zones are formed in the spot test, but no zones are formed at 103, 104, 105, 106...
23 March 2025 308 1 View
What clinical sample extraction procedures do you usually use for metagenomic sequencing analysis? I have read some papers that use filtration, centrifugation, and nuclease digestion... is it...
20 March 2025 9,138 3 View
So I am making polypyrrole from pyrrole using FeCl3 as an oxidant. The end result is a suspension without any precipitate, and the color has changed to black. Is this a good indication that...
18 March 2025 1,613 1 View
Hi Everyone, I am performing free radical polymerization of MMA using AIBN as an initiator and DMF as a solvent (I NEED TO USE A SOLVENT AS IT IS REQUIRED OF THE PROJECT NEXT STEP). I varied AIBN...
14 March 2025 2,927 4 View
The differences in understory vegetation diversity and composition between monoculture and mixed plantations of hybrid poplar and spruce have significant implications for ecosystem processes....
24 February 2025 6,277 7 View
Hello everyone, I have been trying to clone some genes into vector plasmids i have created. using a single restriction enzyme to digest my circular plasmid. I am following a path like " restrict...
16 February 2025 9,685 3 View
I am working with a lncRNA that has multiple isoforms, and I want to determine which are expressed in endothelial cells. Given the typically low expression levels of lncRNAs, my supervisor...
12 February 2025 9,367 0 View
Hi Researchers, I am seeking guidance on how to delete an existing restriction enzyme site and insert a new one in the pcDNA plasmid. My understanding is that I would perform PCR using primers...
04 February 2025 9,137 1 View
I made Zr-based MOF. One is normal, one is just functionalized with -NH2. After analysis, the Isotherm of the normal one is LANGMUIR, the one finctionalized with -NH2 is following FREUNDLICH. Is...
05 January 2025 3,925 3 View
When using gaussian software for opt calculations, how do I fix the central position of the molecule (where there are no atoms, such as ethane), or limit the molecule to a local region of...
27 December 2024 7,002 3 View
I have some old Chitosan sample that I was going to use for research but after FTIR of this sample and comparing it with the standard FTIR spectrum of Chitosan, some of the peaks are missing....
26 December 2024 6,737 3 View
I need to perform genetic distance computation to perform PCoA. I am trying to undertand GenAlEx tutorials but they don't have a tutorial on how to further process dna sequences into the input...
11 December 2024 5,047 0 View
Hi, I work with needle biopsy tissue from the lung and the starting tissue core I get is very small. I've been using the Qiagen Allprep DNA/RNA extraction kit (column base) and I am wondering if...
06 December 2024 5,896 1 View
I have tried working with MelonnPan ( I could not figure out how to prepare the input file), PRISM (site is not accessible) and Metabgc (lack command line to work with it). please suggest some...
13 November 2024 4,440 1 View
I have worked with a protocol to extract them from samples stored in DNA/RNA shield but I am having problems with extracting them from RNA later, any suggestions ? Thank you !
11 November 2024 3,580 0 View
Dear All, We are looking to buy a sequencer for NGS metagenomic analysis and whole genome sequencing. We would really like to get your feedback and user experience on which is better? No doubt...
07 August 2024 2,268 2 View
Hello! I need your help.
01 July 2024 9,032 0 View
what is the optical band gap of pure cobalt oxide nanoparticles? What is the reason for the blue shift observed in the UV-visible spectrum?
27 May 2024 8,326 2 View
Is nuclear power the future of energy and what are the future predictions for nuclear energy and nuclear energy is the hope of future in India?
22 May 2024 1,054 6 View
I deposited TiO2 thin film on Si substrate from a TiO2 oxide target using rf magnetron sputtering technique (only Ar plasma is used ). Can i expect individual Ti and O2 atoms are sputtered from...
20 May 2024 2,310 6 View
can u suggest research frame work using ahp research method
11 May 2024 6,192 2 View