Dear All,
We are looking to buy a sequencer for NGS metagenomic analysis and whole genome sequencing. We would really like to get your feedback and user experience on which is better?
No doubt Illumina has been in the market for a long time hence has a bigger user database, but our budget is limited. The machine we can afford is from Illumina iSec100 or from Nanopore PS solo2 (promethion).
We are working in antibiotic resistance in wastewater and sewer system.
Any response would be very helpful for us.
Thanks in advance
Kanchan
In my experience, generally Illumina is easier to set up and use for independent labs. Is a 1.2G output enough for your work? Is accuracy more important than read length? If yes, choose Illumina.
Unless you are sequencing something on regular basis, I don't think buying the above mentioned sequencers is specially helpful.
Illumina system you mentioned not only generate low amount of data, the read lengths are short. In any lab, there need to be specific someone to operate and maintain the seq platform, which will add additional cost for personal and space.
In case of Nanopore, if not used very frequent, the data generated is not cheaper and data quality is not relatively good as compared to Illumina.
Buying a seq service is a better option if the sequencing is not don't frequently or sample numbers are big.
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