I am working with a lncRNA that has multiple isoforms, and I want to determine which are expressed in endothelial cells. Given the typically low expression levels of lncRNAs, my supervisor suggested using RACE-PCR as a method to enrich the target lncRNA before sequencing with Nanopore.

However, since traditional RACE-PCR only amplifies the 5' and 3' ends separately, I am unsure whether this approach would actually help in obtaining full-length transcripts for sequencing.

My main questions are:

I would appreciate any insights or references on this approach!