Hi,
I work with needle biopsy tissue from the lung and the starting tissue core I get is very small. I've been using the Qiagen Allprep DNA/RNA extraction kit (column base) and I am wondering if I am losing material in the column and should I switch to using a magnetic bead based method for extraction to increase my yield. I often would need both DNA and RNA for downstream whole exome seq and RNA seq. Does anyone have experience working small core biopsies or have done a comparison between using column based and magnetic bead based extraction? I would like to know your experience using either type of extraction method and the quality and yield of the DNA and RNA. Thank you!
Dawn Lau Hi Sir, I have experience in both manual and automated extraction processes in the field of molecular diagnostics. We have been using Zybio (magnetic bead extraction with proteinase k), which provides good results, and the internal control shows a strong curve in RT-PCR. However, the manual process takes more time and involves less error handling. Occasionally, mishandling can lead to internal control errors. There are also issues related to the continuous freezing and thawing of Poly-A (Covid 19 and HCV detection we extract both RNA and DNA) thank you sir
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Materials Science
- Materials
- Nanomaterials