Why does phage titre on double layer agar but not on spot test?

23 March 2025 1 260 Report

I have isolated phage against Y. ruckeri. phages titre 108 pfu/ml on double layer agar, while 101 and 102 pfu/ml zones are formed in the spot test, but no zones are formed at 103, 104, 105, 106 pfu/ml. what is the reason for this?

Isaac Isabirye

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