Metodology for "braking" the aglomerate formed by Azospirillum in liquid medium?

29 August 2024 0 4K Report

To determine efficiently the UFC/ml, I already used the saline solution and had no effect.

Badges
Science topic

More Italo Alves Freire's questions See All

Regarding a model for simulating battery charge and discharge, what do you consider to be high fidelity?

Regarding a model for simulating battery charge and discharge, what do you consider to be high fidelity? What is the acceptable percentage of error (regardless of the metric)? Could you suggest...

03 August 2024 5,358 0 View

Suggestions for semi industrial bioreactor's production papers?

I need information about scale bioreactor's production of bacteria.

27 January 2024 4,013 1 View

Do you think that is intresting to modify a media to better undestand the potential of bacteria to produce organic acids?

I intend to modify a media, to better access de potential of bacteria (Pseudomonas and Bacillus) to produce organic acids in solid medium.

25 January 2024 2,775 1 View

Abaqus error rfm_ArrayRepr::rfm_ArrayRepr when reading .ODB file?

Dear colleagues, I have a python script to read results from ODBs in Abaqus. More specifically, I am extracting the equivalent plastic strain PEEQ from different regions (sets previously...

28 November 2023 3,311 4 View

Could you recommend articles on the wear (aging) of rolls in the steelmaking process?

I am assisting some students with the use of artificial intelligence to measure the wear of rolls used in galvanization. In this context, I encounter two types of rolls: cooled and brush rolls....

28 November 2023 7,794 0 View

How I can resuspend nanoparticles of ZnO pure and doped with Co, Cu and Mn in powder form?

Water not functioned and a solution with the stabilizing agent that I use (MSA = Mercaptosuccinic acid), with a pH ajusted, also not functioned.

01 September 2022 9,948 0 View

Is it possible to publish a book by a friend of mine Giovanni Filocamo who died in 2016?

The painter Giovanni Filocamo, known as Vanni, was born in 1937 in Reggio Calabria. He attends the art school directed by Alfonso Frangipane and devotes himself to teaching "ornate" and "drawn...

24 March 2022 2,367 4 View

How open .czi file in ImageJ or Fiji?

Dear all, I have some .czi files (image format from Zeiss cameras) to analyse (particle counting, area and so on). I have tried several times to open them with the most recent versions of...

09 October 2021 5,044 3 View

Which is the best software to facilitate the quantification of maxN, richness and total length in fish communities with mono-BRUVs videos?

In my laboratory, we are new to the use of BRUVs to obtain the abundance, richness, and biomass parameters of reef fish communities. We want to know if any software allows us to record the...

09 September 2021 4,190 1 View

Do you know some methodology of synthesis current of nanoparticles of ZnO in an aqueous medium?

Is for the data survey of my research of master which involves firstly the synthesis of nanoparticles of ZnO in colloidal systems in an aqueous medium. Thank you since now!

14 April 2021 178 9 View

What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

07 August 2024 613 3 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

How can we conduct DFT for solutions or mixtures ?

Can we conduct DFT studies for Ionic Liquids with Amines at various concentrations? If so, how can we proceed with these studies? Additionally, what parameters, besides IR, NMR, and HOMO-LUMO, can...

18 July 2024 319 1 View

Has anyone tried freeze flow samples in -80C freezer or liquid nitrogen before processing?

Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...

15 July 2024 9,975 1 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,898 0 View

Why am I seeing only one initial maximum in torque-time curves for the fusion of PVC in a Brabender?

We're studying the stability and fusion behavior of multiple PVC compounds in a Brabender. After a few trials, we set the temperature at 195 °C and the rotor speed at 70 rpm. Samples differ in...

12 July 2024 2,768 1 View