How to do multiple docking in a single protein ?

More Pratyasha Bhowal's questions See All

What is the role of cut-off wavelength in fluorescence measurement?

I am using the filter pair of 480/535 and trying to measure fluorescence. The instrument that I am using is Spectramax M2. The Cut-off filter for this pair is showing 530nm in this machine. So...

27 May 2020 691 3 View

How to quantify protein content in an enzyme having a unit of 1000 U/ml?

I am trying to quantify the protein in terms of mg/ml by BCA assay with an activity of 1000 u/ml.

23 December 2019 4,389 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

What are the potential shell well and atomic solvation parameters for the boron atom?

I have a protein-ligand docking project. The ligand contains boron atoms that are not detected by Autodock software. To solve this problem, it is necessary to introduce the boron atom to...

19 February 2021 1,236 1 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

The best RNA isolation kit for lncRNA RNA-Seq?

Hi, I am preparing a new project on lncRNAs in human cancer cell lines. I scanned through many high-impacted journals to find the best RNA isolation kit that would also isolate small RNAs and that...

09 February 2021 4,180 5 View

How to Remove different version of atom from pdb Crystal Structure for Docking Preparation?

I am new to Docking and MD, and currently try to catch on it. I try one of the webserver for docking, but unfortunately not working. it says " Your PDB contains multiple forms of the same residue...

08 February 2021 7,426 3 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

What could be the possible causes of DNA degradation after extraction from 4% gel?

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02 February 2021 5,008 2 View

How does DMSO percentage affect ELISA?

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31 January 2021 6,771 1 View

Can I heat my ELISA at 37C?

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31 January 2021 4,271 3 View

Jobin Thomas

You can use Autodock Vina.

Mohamed asif Shajahan

I agree with Mr. Jobin Thomas , but putting it into practise would be the most challenging aspect.

so you can try EasyDockVina2 for multiple ligand docking

Code EasyDockVina2: Graphical User Interface for Ligand Optimizat...

Amanpreet Kaur

You can use autodock vina or pyrx for multiple docking

Pratyasha Bhowal

Thank you so much. I have been able to do it.