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How can I measure the thickness of thin film by Uv-Vis?
07 August 2024 7,769 3 View
I want to remove Urea from my protein (6kDa) sample. I did use : 1. Step down the Urea gradient (dialysis) in order to remove urea and refold my protein, but my protein still contains urea in the...
07 August 2024 1,291 4 View
Greetings When one is to perform Simulation or modelling of Pyrolysis of Biomass Waste materials using Aspen Plus software, and one has both the macromolecules breakdown (Cellulose, Lignin and...
07 August 2024 4,978 1 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
07 August 2024 931 5 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
07 August 2024 757 3 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
07 August 2024 610 4 View
Are there any suggestions or insights you can provide to help address this problem
07 August 2024 1,391 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
07 August 2024 1,434 2 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
07 August 2024 599 2 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
07 August 2024 3,084 2 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
07 August 2024 1,322 3 View
Dear friends, does anybody know that is it legal to upload a graphical abstract previously published on your paper's first page to a website such as figshare.com under CC-BY-4.0 license? Thanks.
06 August 2024 7,040 3 View
Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?
06 August 2024 2,279 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
06 August 2024 9,102 4 View
The political participation of the Kirat Rai community in Nepal has evolved significantly over time: Historical Context Historically, the Kirat Rai people, like many indigenous groups in Nepal,...
06 August 2024 5,916 1 View
Do experts have journals in the field of artificial intelligence and big data that are not indexed by SCI or EI?
06 August 2024 8,791 2 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
06 August 2024 9,475 2 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
06 August 2024 4,902 3 View
Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...
06 August 2024 1,176 2 View
My case has been under treatment now for 3 years with little response. Now considering euthanasia. Last ditch is Cisplatin for dilution in iv infusion (100ml/ml in saline and mannitol). What can I...
06 August 2024 1,785 0 View
I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...
06 August 2024 5,265 0 View
Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...
06 August 2024 3,004 6 View
I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II
06 August 2024 5,238 2 View
I attempted to make a privately uploaded text public but a window appeared that said an error occurred. There was no explanation provided as to why there was an error or what might be done to...
06 August 2024 7,982 7 View