I want to work on butterflies, and need your suggestion on the methodology to be used, what to focus on, what should be my objectives and aim of my studies, it's just for a period of about a month. It is a mini project for an undergraduate.
Transects: 3–5 fixed 200–500 m routes (“Pollard Walks”). Mark them on a map.
Frequency: Survey each transect 2 times/day (late morning & early afternoon), 3–4 days/week for 4 weeks. Avoid rain during active counts; note if it rained earlier.
Effort control: Walk each transect in ~15–20 min at constant pace; 5 m detection band.
Weather logging: At start & end of each transect (or every 10 min) record: air temp, wind (Beaufort or meter), RH, % cloud/solar (simple 0–8 oktas), and whether it rained in last 24–72 h. Use a handheld meter if possible; otherwise, combine a shaded thermometer + nearby weather-station data.
Species data: Count individuals by species where possible; if ID is tough, group into guilds (e.g., swallowtails, whites, nymphalids).
Covariates (quick): time of day, microhabitat (edge/open/shade), flowers in bloom (0–3 scale), observer.
Simple data sheet (columns)
date | site | transect | start_time | end_time | duration_min | temp_C | wind_ms | RH_% | cloud_oktas | rain_24h (0/1) | habitat | flower_score | species | count
Analysis (straightforward)
Exploration: scatterplots of counts vs temp; boxplots by cloud class; line of activity across time of day.
Model(s): counts are typically overdispersed → use Negative Binomial (or Poisson if variance≈mean).
If multiple transects/sites: add Transect (random effect) → GLMM.
Thresholds: Fit a quadratic (Temp + Temp²) or a segmented regression to estimate optimum temperature.
Outputs to report: effect sizes (β), 95% CIs, pseudo-R², and predicted activity vs temperature curve at low vs high wind.
Sampling schedule you can finish in a month
Week 1: pilot the routes, practice IDs, finalize datasheet.
Weeks 2–3: full sampling (aim for ≥18–24 transect walks total).
Week 4: data cleaning, plots, model, write-up.
Interpreting “expansion”
In one month you can’t track range expansion, but you can infer favorable expansion conditions by identifying weather windows with peak activity and movement (e.g., sunny, warm, low-wind afternoons). Discuss how these conditions enhance dispersal (warmer thoracic temps → longer flights; calm air → straighter movement; post-rain nectar → fueling flights).
Low-cost equipment & tips
Clipboard, printed datasheets, stopwatch/phone timer, simple thermometer, wind meter (or Beaufort scale chart), RH meter if available, camera for uncertain IDs.
Standardize clothing color and pace to reduce detection bias.
Ethical note: no capture needed; if doing mark-release-recapture for movement, use non-toxic fine marker on the wing margin—only if you’re trained and permitted.
Deliverables (what to hand in)
1–2 pages methods with a site map and transect GPS traces.
Figures: (i) temp vs predicted activity curve, (ii) bar/line showing wind or cloud effects, (iii) heat table of species × weather class.
Short discussion linking mechanisms (thermoregulation, nectar availability, flight costs) to your results and to practical survey guidance (e.g., “Survey between 10:30–14:30 when temp 28–33 °C, wind
Excellent answer! Butterflies can be warmed by solar radiation, so it's worth measuring the operative and ambient temperature in the sun, as well as the ambient temperature in the shade. The operative and ambient temperatures in the sun can be measured using a hair-fine thermocouple (so fine that it doesn't decrease its reading when you move it from full sun into the shade) - inserted into a dead butterfly in the basking position, for the operative temperature.