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I introduced a point mutation in my protein and cloned the mutated gene into the pCold vector. After sequencing, I observed a gap of 2 base pairs upstream of the mutation site. Considering the gap...
20 June 2025 422 3 View
Is it possible to non-experimentally determine the critical denaturation temperature, by which only (or mostly) mutant DNA is melted during a fast COLD-PCR assay or a classical COLD-PCR assay? If...
20 June 2025 1,793 0 View
Request for Input from Fellow Researchers Hello, I’m currently working on extracting paraquat from agricultural soil in order to quantify it using the ascorbic acid method with UV-Vis...
19 June 2025 1,097 3 View
Device structure:ITO/PEDOT:PSS/PVSK/MoO3/AG After testing and analyzing the JV curves, it was found that most of them were a single diagonal line (n=1), and only two were relatively in line with...
18 June 2025 2,528 0 View
How do the mechanical and thermal insulation properties of arbolite concrete compare to conventional concrete, and what are the primary factors influencing its performance in structural and...
17 June 2025 2,135 1 View
Minha questão refere-se ao convite de João XXIII ao discernimento dos sinais dos tempos. Este termo é usado originalmente por Jesus no Evangelho. Ao usá-lo o Papa João XXIII convocou a Igreja...
17 June 2025 6,505 2 View
Dear all, I read that a 21-point digital version of the Self-Assessment Manikin (SAM) Scale exists, but I haven't been able to find any visual examples or detailed descriptions of its...
17 June 2025 122 0 View
India ranks 3rd in the Asian region for scientific research in 2024, demonstrating a strong output with 1167 documents and 1056 citable documents. Its H-index of 101 further solidifies its...
16 June 2025 9,803 0 View
With increase in recent accidents including Air, Rail, Road accidents increasing. Latest Data reveals still Air is far safer than RTA, Road traffic accidents (about 400 people die of RTA daily in...
15 June 2025 5,767 5 View
Dear all, I have problem on peak recognition during PFAS quantification. Please check the attachment photo, should I recognise as PFAS compound or only as contaminant? There are any rule for peak...
13 June 2025 9,362 1 View
Can exposure to information be treated as situational factors? Should I consider it as micro environmental factor? Also suggestion for indicators to measure the exposure to information from the...
13 June 2025 3,065 0 View
Are you tired of spending hours searching for the right journal for your research paper, only to face rejections? We understand the struggle! Choosing the best journal is critical, yet many...
11 June 2025 4,974 0 View
i need to compare on how to draft the empirical theory
11 June 2025 6,724 1 View
The quality of our DNA electrophoresis (for genotyping) varies from experiment to experiment, and I am trying to understand why. Sometimes bands look crisp and sharp, and other times they have a...
10 June 2025 607 4 View
Why was the prevalence in this study categorized based on FEC (fecal egg count) into low (0–199 EPG), medium (200–500 EPG), and high (>500 EPG) for strongyle infection? Is there a specific...
09 June 2025 4,999 0 View
We are currently investigating the presence of microplastics in dairy products using chemical digestion methods. However, identifying microplastic particles under a standard optical microscope...
08 June 2025 386 1 View
The pathogen Colletotrichum lagenarium, along with other similar fungi, causes anthracnose in mirlitons (chayote) in the United States. Most growers have found that existing fungicides are...
07 June 2025 2,716 2 View
management, a toxic work environment is a factor that negatively impacts the quality of professional performance. Aggressive or negative behaviors from colleagues or management may prevail,...
05 June 2025 4,224 1 View
I'm performing a Luciferase assay using 1X RLB on HeLa cells plated in a 24-well plate. I've tried the lysis multiple times, even switching to a fresh buffer and extending the lysis time on ice,...
05 June 2025 3,611 1 View
Goal: I want to observe the expression level of genes (A and B) in a mutant (mt) vs. WT bacteria with/without phage infection. Hypothesis: The Gene A or B expression level in the two bacterial...
05 June 2025 6,587 4 View
What is the impact of ANOVA in Science of data?
05 June 2025 8,241 4 View
When measuring the radiation pattern in the E-plane and H-plane, it's common to rotate the measurement setup by 90° to capture the next plane. Now, consider a rectangular waveguide antenna...
03 June 2025 1,856 1 View
Can laser cutting or CNC punching on pre-swollen membranes improve hole alignment accuracy in RED stack assembly compared to manual punching, or are there other effective methods available for...
03 June 2025 7,397 3 View
I NEED ABOUT 50 GRAMS OF ULTRAHIGH MOLECULAR WEIGHT POLYETHYLENE NANOPARTICLE FOR WEAR PARTICLE-INDUCED PERIPROSTHETIC OSTEOLYSIS RESEARCH.
02 June 2025 997 3 View